Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kuliopulos, A.; Nelson, N.P.; Yamada, M.; Walsh, C.T.; Furie, B.; Furie, B.C.; Roth, D.A.
    Localization of the affinity peptide-substrate inactivator site on recombinant vitamin K-dependent carboxylase (1994), J. Biol. Chem., 269, 21364-21370.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.90 expressed in baculovirus-infected insect cell. Produced His6-tagged carboxylase as a recombinant protein using a baculovirus expression system Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.90 bromoacetyl-FLEEL peptide the His6-carboxylase is irreversibly inactivated. Up to 85% of the carboxylase activity is lost over a period of 120 min Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.90 1.5
-
 FLEEL wild-type carboxylase in bovine liver microsomes Bos taurus
4.1.1.90 1.6
-
 FLEEL recombinant His6-carboxylase present in Sf9 cell microsomes Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.90 microsome
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.90 94000
-
 SDS-PAGE and Western Blot, His6-carboxylase-Ac-FLEEL confirming affinity-purified carboxylase Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone Bos taurus
-
 gamma-carboxy-L-glutamate + vitamin K epoxide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.90 Bos taurus Q07175
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.90 inactivated His6-carboxylase-Ac-FLEEL purified under denaturing conditions by Ni-chelation chromatography followed by preparative polyacrylamide gel electrophoresis Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.90 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.90 FLEEL + CO2 + O2 + vitamin KH2
-
 Bos taurus ? + vitamin K epoxide + H2O
-
 ?
4.1.1.90 L-glutamate + CO2 + O2 + vitamin K hydroquinone
-
 Bos taurus gamma-carboxy-L-glutamate + vitamin K epoxide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.90 monomer
-
 Bos taurus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.90 gamma-glutamyl carboxylase
-
 Bos taurus
4.1.1.90 glutamate carboxylase
-
 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.90 1
-
 FLEEL wild-type carboxylase in bovine liver microsomes Bos taurus
4.1.1.90 1.7
-
 FLEEL recombinant His6-carboxylase present in Sf9 cell microsomes Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.90 vitamin K
-
 Bos taurus