Literature summary extracted from
Berg, A.; Ingelman-Sundberg, M.; Gustafsson, M.
Purification and characterization of cytochrome P-450meg (1979), J. Biol. Chem., 254, 5264-5271.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.14.15.8 |
phosphatidylcholine |
stimulates |
Priestia megaterium |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.14.15.8 |
52000 |
- |
x * 52000, SDS-PAGE |
Priestia megaterium |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.15.8 |
Priestia megaterium |
Q06069 |
ATCC 13368 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.15.8 |
the purification procedure includes chromatography on DEAE-cellulose, Ultrogel ACA-54, DEAE-Sepharose, octyl-Sepharose, and hydroxyapatite |
Priestia megaterium |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.14.15.8 |
0.0008 |
- |
- |
Priestia megaterium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.15.8 |
progesterone + reduced ferredoxin + O2 |
Bacillus megaterium ferredoxin may be replaced by adrenal ferredoxin |
Priestia megaterium |
15beta-hydroxy-progesterone + oxidized ferredoxin + H2O |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.15.8 |
? |
x * 52000, SDS-PAGE |
Priestia megaterium |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.15.8 |
cytochrome P-450meg |
- |
Priestia megaterium |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.15.8 |
22 |
- |
assay at |
Priestia megaterium |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.15.8 |
7.4 |
- |
assay at |
Priestia megaterium |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.15.8 |
NADPH |
- |
Priestia megaterium |
|
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
1.14.15.8 |
Priestia megaterium |
isoelectric focusing |
- |
4.9 |