EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.74 | iodoacetamide | 5 mM, 16% inhibition | Sus scrofa | |
4.2.1.74 | N-Methylmaleimide | 1 mM, 69% inhibition, substrate: trans-octenoyl-CoA | Sus scrofa | |
4.2.1.74 | p-chloromercuribenzoate | 1 mM, complete inhibition | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.74 | 0.024 | - |
trans-2-decenoyl-CoA | 25°C | Sus scrofa | |
4.2.1.74 | 0.024 | - |
trans-2-dodecenoyl-CoA | 25°C | Sus scrofa | |
4.2.1.74 | 0.024 | - |
trans-2-octenoyl-CoA | 25°C | Sus scrofa | |
4.2.1.74 | 0.024 | - |
trans-2-tetradecenoyl-CoA | 25°C | Sus scrofa | |
4.2.1.74 | 0.045 | - |
trans-2-hexenoyl-CoA | 25°C | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.74 | mitochondrion | - |
Sus scrofa | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.74 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.74 | partial | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.1.74 | heart | - |
Sus scrofa | - |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.74 | - 20°C, stable for several months | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.74 | additional information | activity with crotonyl-CoA is 1% of the activity with trans-decenoyl-CoA | Sus scrofa | ? | - |
? | |
4.2.1.74 | trans-2-decenoyl-CoA + H2O | Vmax is 74% of Vmax for trans-octenoyl-CoA | Sus scrofa | (3S)-3-hydroxydecanoyl-CoA | - |
? | |
4.2.1.74 | trans-2-dodecenoyl-CoA + H2O | Vmax is 42% of Vmax for trans-octenoyl-CoA | Sus scrofa | (3S)-3-hydroxydodecanoyl-CoA | - |
? | |
4.2.1.74 | trans-2-hexenoyl-CoA + H2O | Vmax is 78% of Vmax for trans-octenoyl-CoA | Sus scrofa | (3S)-3-hydroxyhexanoyl-CoA | - |
? | |
4.2.1.74 | trans-2-octenoyl-CoA + H2O | best substrate | Sus scrofa | (3S)-3-hydroxyoctanoyl-CoA | - |
? | |
4.2.1.74 | trans-2-tetradecenoyl-CoA + H2O | Vmax is 34% of Vmax for trans-octenoyl-CoA | Sus scrofa | (3S)-3-hydroxytetradecanoyl-CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.74 | long chain enoyl coenzyme A hydratase | - |
Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.74 | 8.5 | - |
- |
Sus scrofa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.74 | 7 | 9 | pH 7: about 50% of maximal activity, pH 9: 80% of maximal activity | Sus scrofa |