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Literature summary extracted from

  • Mogi, T.; Murase, Y.; Mori, M.; Shiomi, K.; Omura, S.; Paranagama, M.P.; Kita, K.
    Polymyxin B identified as an inhibitor of alternative NADH dehydrogenase and malate: quinone oxidoreductase from the Gram-positive bacterium Mycobacterium smegmatis (2009), J. Biochem., 146, 491-499.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.4 (S)-malate in the presence of polymyxin B, enzyme kinetics changes from the Michaelis-Menten type to substrate inhibition kinetics with the substrate inhibition constant Ksi of 57.4 microg/ml Mycolicibacterium smegmatis
1.1.5.4 nanaomycin A naphthoquinone derivative Mycolicibacterium smegmatis
1.1.5.4 Polymyxin B cationic decapeptide. Primary site of action is the quinone-binding site, amino acid sequence is examined and possible binding sites for L-malate and quinones are found Mycolicibacterium smegmatis
7.1.1.2 nanaomycin A
-
 Mycolicibacterium smegmatis
7.1.1.2 Polymyxin B in the presence of polymyxin B, enzyme kinetics changes from the Michaelis–Menten type to substrate inhibition kinetics with the substrate inhibition Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.4 0.015
-
 ubiquinone-1 pH 7.4, 25°C Mycolicibacterium smegmatis
1.1.5.4 2.6
-
 (S)-malate pH 7.4, 25°C Mycolicibacterium smegmatis
7.1.1.2 0.015
-
 ubiquinone-1 pH 7.4, 25°C Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 membrane
-
 Mycolicibacterium smegmatis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.4 Mycolicibacterium smegmatis
-
-
-
7.1.1.2 Mycolicibacterium smegmatis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.4 (S)-malate + ubiquinone-1
-
 Mycolicibacterium smegmatis oxaloacetate + ubiquinol-1
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-1
-
 Mycolicibacterium smegmatis NAD+ + ubiquinol-1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.4 malate:quinone oxidoreductase
-
 Mycolicibacterium smegmatis
1.1.5.4 malate:quinone reductase
-
 Mycolicibacterium smegmatis
1.1.5.4 MQR
-
 Mycolicibacterium smegmatis
7.1.1.2 NDH2
-
 Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.4 25
-
 assay at Mycolicibacterium smegmatis
7.1.1.2 25
-
 assay at Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.4 7.4
-
 assay at Mycolicibacterium smegmatis
7.1.1.2 7.4
-
 assay at Mycolicibacterium smegmatis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.5.4 additional information
-
 additional information in the presence of polymyxin B, enzyme kinetics changes from the Michaelis-Menten type to substrate inhibition kinetics with the substrate inhibition constant Ksi of 57.4 microg/ml. Polymyxin B inhibits the malate-dependent reaction noncompetitively with the Ki value of 7.0 microg/ml Mycolicibacterium smegmatis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.5.4 additional information
-
 IC50 for polymyxin B is 4.2 microg/ml, IC50 for nanaomycin is 49 microg/ml Mycolicibacterium smegmatis additional information
7.1.1.2 additional information
-
 IC50 for polymyxin B is 0.0014 mg/ml, IC50 for nanaomycin A is 0.031 mg/ml Mycolicibacterium smegmatis additional information