BRENDA - Enzyme Database show

In vitro and in vivo interactions of ferredoxin-NADP+ reductases in Pseudomonas putida

Yeom, J.; Jeon, C.O.; Madsen, E.L.; Park, W.; J. Biochem. 145, 481-491 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.18.1.2
expressed via plasmid in Escherichia coli strain BL21(DE3)
Pseudomonas putida
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.2
0.00083
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
0.00111
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00112
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00181
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
0.00191
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor; for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00245
-
NADPH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
0.00263
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00298
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00326
-
NADPH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
0.00397
-
NADPH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
0.00464
-
NADH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
0.00479
-
NADPH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
0.00615
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
0.00686
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
0.00717
-
NADH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
0.00802
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
0.00822
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
0.00933
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00943
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
0.01063
-
NADH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
0.01163
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
0.02363
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
0.0632
-
NADH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.1.2
27000
-
SDS-PAGE
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.2
[4Fe-4S]-ferredoxin + NADPH
Pseudomonas putida
[4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld)
reduced [4Fe-4S]-ferredoxin + NADP+
-
-
r
1.18.1.2
[4Fe-4S]-ferredoxin + NADPH
Pseudomonas putida KT 2240
[4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld)
reduced [4Fe-4S]-ferredoxin + NADP+
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.1.2
Pseudomonas putida
-
wild type and mutants fprA (lacking FprA) and fprB (lacking FprB)
-
1.18.1.2
Pseudomonas putida KT 2240
-
wild type and mutants fprA (lacking FprA) and fprB (lacking FprB)
-
Purification (Commentary)
EC Number
Commentary
Organism
1.18.1.2
-
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.2
NADH + K3Fe(CN)6
-
698661
Pseudomonas putida
NAD+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
NADH + K3Fe(CN)6
-
698661
Pseudomonas putida KT 2240
NAD+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida
NADP+ + reduced 2,6-dichlorophenolindophenol
-
-
-
r
1.18.1.2
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida KT 2240
NADP+ + reduced 2,6-dichlorophenolindophenol
-
-
-
r
1.18.1.2
NADPH + K3Fe(CN)6
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida
NADP+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
NADPH + K3Fe(CN)6
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida KT 2240
NADP+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
oxidized 2,6-dichlorophenolindophenol + NADH
-
698661
Pseudomonas putida
NAD+ + reduced 2,6-dichlorophenolindophenol
-
-
-
r
1.18.1.2
oxidized 2,6-dichlorophenolindophenol + NADH
-
698661
Pseudomonas putida KT 2240
NAD+ + reduced 2,6-dichlorophenolindophenol
-
-
-
r
1.18.1.2
oxidized ferredoxin + NADH + cytochrome c
-
698661
Pseudomonas putida
reduced ferredoxin + NAD+ + reduced cytochrome c
-
-
-
r
1.18.1.2
oxidized ferredoxin + NADPH + cytochrome c
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida
reduced ferredoxin + NADP+ + reduced cytochrome c
-
-
-
r
1.18.1.2
[4Fe-4S]-ferredoxin + NADPH
[4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld)
698661
Pseudomonas putida
reduced [4Fe-4S]-ferredoxin + NADP+
-
-
-
r
1.18.1.2
[4Fe-4S]-ferredoxin + NADPH
[4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld)
698661
Pseudomonas putida KT 2240
reduced [4Fe-4S]-ferredoxin + NADP+
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
1.18.1.2
monomer
1 * 27000, fast protein liquid chromatography
Pseudomonas putida
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.18.1.2
1.16
-
NADH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
3.03
-
NADH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
3.09
-
NADH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
3.82
-
NADPH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
4.51
-
NADPH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
4.59
-
NADH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
4.77
-
NADPH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
5.99
-
NADPH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.1.2
expressed via plasmid in Escherichia coli strain BL21(DE3)
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.2
0.00083
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
0.00111
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00112
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00181
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
0.00191
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor; for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00245
-
NADPH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
0.00263
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00298
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00326
-
NADPH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
0.00397
-
NADPH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
0.00464
-
NADH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
0.00479
-
NADPH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
0.00615
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
0.00686
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
0.00717
-
NADH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
0.00802
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
0.00822
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
0.00933
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
0.00943
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
0.01063
-
NADH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
0.01163
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
0.02363
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
0.0632
-
NADH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.1.2
27000
-
SDS-PAGE
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.2
[4Fe-4S]-ferredoxin + NADPH
Pseudomonas putida
[4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld)
reduced [4Fe-4S]-ferredoxin + NADP+
-
-
r
1.18.1.2
[4Fe-4S]-ferredoxin + NADPH
Pseudomonas putida KT 2240
[4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld)
reduced [4Fe-4S]-ferredoxin + NADP+
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.1.2
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.2
NADH + K3Fe(CN)6
-
698661
Pseudomonas putida
NAD+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
NADH + K3Fe(CN)6
-
698661
Pseudomonas putida KT 2240
NAD+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida
NADP+ + reduced 2,6-dichlorophenolindophenol
-
-
-
r
1.18.1.2
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida KT 2240
NADP+ + reduced 2,6-dichlorophenolindophenol
-
-
-
r
1.18.1.2
NADPH + K3Fe(CN)6
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida
NADP+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
NADPH + K3Fe(CN)6
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida KT 2240
NADP+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
oxidized 2,6-dichlorophenolindophenol + NADH
-
698661
Pseudomonas putida
NAD+ + reduced 2,6-dichlorophenolindophenol
-
-
-
r
1.18.1.2
oxidized 2,6-dichlorophenolindophenol + NADH
-
698661
Pseudomonas putida KT 2240
NAD+ + reduced 2,6-dichlorophenolindophenol
-
-
-
r
1.18.1.2
oxidized ferredoxin + NADH + cytochrome c
-
698661
Pseudomonas putida
reduced ferredoxin + NAD+ + reduced cytochrome c
-
-
-
r
1.18.1.2
oxidized ferredoxin + NADPH + cytochrome c
assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH
698661
Pseudomonas putida
reduced ferredoxin + NADP+ + reduced cytochrome c
-
-
-
r
1.18.1.2
[4Fe-4S]-ferredoxin + NADPH
[4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld)
698661
Pseudomonas putida
reduced [4Fe-4S]-ferredoxin + NADP+
-
-
-
r
1.18.1.2
[4Fe-4S]-ferredoxin + NADPH
[4Fe-4S]-ferredoxin of Pseudomonas putida is similar to ferredoxin FdI of Azotobacter vinelandii, the Pseudomonas putida chromosome contains two [2Fe-2S] ferredoxins (FdA and FdB), three [4Fe-4S] ferredoxins (4FdA, 4FdB and FdxA) and one flavodoxin (Fld)
698661
Pseudomonas putida KT 2240
reduced [4Fe-4S]-ferredoxin + NADP+
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.18.1.2
monomer
1 * 27000, fast protein liquid chromatography
Pseudomonas putida
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.18.1.2
1.16
-
NADH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
3.03
-
NADH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
3.09
-
NADH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
3.82
-
NADPH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
4.51
-
NADPH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
4.59
-
NADH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
4.77
-
NADPH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
5.99
-
NADPH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.18.1.2
70
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
90
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor; for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
110
-
NADH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
130
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
170
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
530
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
540
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
680
-
NADH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
770
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
860
-
NADH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
1000
-
NADPH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
1130
-
NADPH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
1140
-
NADPH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
1370
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
1860
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
3050
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
3230
-
NADPH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
3720
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
4590
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
4630
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
6730
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
11000
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
11510
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.18.1.2
70
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
90
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor; for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
110
-
NADH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
130
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
170
-
NADH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
530
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
540
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
680
-
NADH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
770
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdB as electron acceptor
Pseudomonas putida
1.18.1.2
860
-
NADH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
1000
-
NADPH
for FprA in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
1130
-
NADPH
for FprB in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
1140
-
NADPH
for FprB in a flavin free assay, using 2,6-dichlorophenolindophenol as electron acceptor
Pseudomonas putida
1.18.1.2
1370
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
1860
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
3050
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
3230
-
NADPH
for FprA in a flavin free assay, using K3Fe(CN)6 as electron acceptor
Pseudomonas putida
1.18.1.2
3720
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida
1.18.1.2
4590
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
4630
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [4Fe-4S]-ferredoxin FdxA as electron acceptor
Pseudomonas putida
1.18.1.2
6730
-
NADH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
11000
-
NADPH
for FprB in a cytochrome c assay, using Pseudomonas putida [2Fe-2S]-ferredoxin FdA as electron acceptor
Pseudomonas putida
1.18.1.2
11510
-
NADPH
for FprA in a cytochrome c assay, using Pseudomonas putida flavodoxin Fld as electron acceptor
Pseudomonas putida