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Literature summary extracted from

  • Toraya, T.; Tamura, N.; Watanabe, T.; Yamanishi, M.; Hieda, N.; Mori, K.
    Mechanism-based inactivation of coenzyme B12-dependent diol dehydratase by 3-unsaturated 1,2-diols and thioglycerol (2008), J. Biochem., 144, 437-446.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.28 expression in Escherichia coli strain JM109 Klebsiella oxytoca

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.28 1,2-Butanediol
-
 Klebsiella oxytoca
4.2.1.28 3-butene-1,2-diol holodiol dehydratase undergoes rapid and irreversible inactivation, the inactivation cleaves the Co-C bond of adenosylcobalamin irreversibly forming unidentified radicals and cob(II)alamin that resist oxidation even in the presence of oxygen, inactivation mechanism, overview Klebsiella oxytoca
4.2.1.28 3-butyne-1,2-diol holodiol dehydratase undergoes rapid and irreversible inactivation, the inactivation cleaves the Co-C bond of adenosylcobalamin irreversibly forming unidentified radicals and cob(II)alamin that resist oxidation even in the presence of oxygen, inactivation mechanism, overview Klebsiella oxytoca
4.2.1.28 additional information inactivated holoenzymes undergo reactivation by diol dehydratase-reactivating factor in the presence of ATP, Mg2+ and adenosylcobalamin Klebsiella oxytoca
4.2.1.28 thioglycerol holodiol dehydratase undergoes rapid and irreversible inactivation, the inactivation cleaves the Co-C bond of adenosylcobalamin irreversibly forming unidentified radicals and cob(II)alamin that resist oxidation even in the presence of oxygen, inactivation mechanism, overview Klebsiella oxytoca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.28 additional information
-
 additional information kinetic analysis with substrate analogues 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol Klebsiella oxytoca
4.2.1.28 0.08
-
 1,2-propanediol pH 8.0, 37°C Klebsiella oxytoca
4.2.1.28 2.5
-
 1,2-Butanediol pH 8.0, 37°C Klebsiella oxytoca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.28 Co2+ the is dependent on coenzyme B12, adenosylcobalamin Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.28 1,2-propanediol Klebsiella oxytoca
-
 propanal + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Klebsiella oxytoca
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.2.1.28 inactivated holoenzymes undergo reactivation by diol dehydratase-reactivating factor in the presence of ATP, Mg2+ and adenosylcobalamin Klebsiella oxytoca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-butanediol
-
 Klebsiella oxytoca butanal + H2O
-
 ?
4.2.1.28 1,2-propanediol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
4.2.1.28 3-butene-1,2-diol
-
 Klebsiella oxytoca ?
-
 ?
4.2.1.28 3-butyne-1,2-diol
-
 Klebsiella oxytoca ?
-
 ?
4.2.1.28 thioglycerol
-
 Klebsiella oxytoca ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.28 coenzyme B12-dependent diol dehydratase
-
 Klebsiella oxytoca
4.2.1.28 diol dehydratase
-
 Klebsiella oxytoca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.28 37
-
 assay at Klebsiella oxytoca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.28 7.8
-
 1,2-Butanediol pH 8.0, 37°C Klebsiella oxytoca
4.2.1.28 366
-
 1,2-propanediol pH 8.0, 37°C Klebsiella oxytoca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.28 8
-
 assay at Klebsiella oxytoca

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 coenzyme B12 adenosylcobalamin, dependent on, two mol of 5'-deoxyadenosine per mol of enzyme are formed as an inactivation product from the coenzyme adenosyl group in presence of inhibitors, overview Klebsiella oxytoca

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.28 additional information
-
 additional information kinetic analysis with inhibitory substrate analogues 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol Klebsiella oxytoca
4.2.1.28 0.02
-
 3-butyne-1,2-diol pH 8.0, 37°C Klebsiella oxytoca
4.2.1.28 0.03
-
 3-butene-1,2-diol pH 8.0, 37°C Klebsiella oxytoca
4.2.1.28 0.1
-
 thioglycerol pH 8.0, 37°C Klebsiella oxytoca
4.2.1.28 1.97
-
 1,2-Butanediol pH 8.0, 37°C Klebsiella oxytoca