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Literature summary extracted from
- Presence of two trans-o-hydroxybenzylidenepyruvate hydratase-aldolases in naphthalenesulfonate-assimilating Sphingomonas paucimobilis TA-2: comparison of some properties (2000), J. Biochem., 127, 43-49.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.2.45 | Hg2+ | 1 mM, 40% inhibition of fHBP HA B | Sphingomonas paucimobilis |  |
| 4.1.2.45 | additional information | no inhibition: monoiodoacetic acid, N-ethylmaleimide, PCMB, diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, EDTA, NaN3, KCN, o-phenanthroline, 2,2'-bipyridine, Fe2+, Fe3+, Mg2+, Mn2+, Co2+, Ca2+, Zn2+, Cd2+, Cu2+, Ni2+, EDTA, o-phenanthroline, 2'-bipyridine, KCN | Sphingomonas paucimobilis | |
| 4.1.2.45 | NEM | 1 mM, 30 min, 25°C, 35% inhibition of fHBP HA B, more than 85% of the initial activity of fHBP HA A remains | Sphingomonas paucimobilis | |
| 4.1.2.45 | PCMB | 1 mM, 30 min, 25°C, complete inhibition of fHBP HA B, more than 85% of the initial activity of fHBP HA A remains | Sphingomonas paucimobilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.45 | 0.003 | - |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate | 30°C, pH 8.1, enzyme form fHBP HA A | Sphingomonas paucimobilis | |
| 4.1.2.45 | 0.009 | - |
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate | 30°C, pH 8.1, enzyme form fHBP HA B | Sphingomonas paucimobilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 37200 | - |
3 * 37200, enzyme form fHBP HA B, SDS-PAGE | Sphingomonas paucimobilis |
| 4.1.2.45 | 37600 | - |
3 * 37600, enzyme form fHBP HA A, SDS-PAGE | Sphingomonas paucimobilis |
| 4.1.2.45 | 104000 | - |
enzyme form fHBP HA B, ultracentrifugation | Sphingomonas paucimobilis |
| 4.1.2.45 | 116000 | - |
enzyme form fHBP HA A, ultracentrifugation | Sphingomonas paucimobilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.45 | Sphingomonas paucimobilis | - |
formerly Pseudomonas sp. TA-2 | - |
| 4.1.2.45 | Sphingomonas paucimobilis TA-2 | - |
formerly Pseudomonas sp. TA-2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.45 | presence of at least three trans-o-hydroxybenzylidenepyruvate hydratase-aldolases, purification of two typical trans-o-hydroxybenzylidenepyruvate hydratase-aldolases (fHBP HA A and fHBP HA B) | Sphingomonas paucimobilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 7.7 | - |
enzyme form fHBP HA B | Sphingomonas paucimobilis |
| 4.1.2.45 | 11 | - |
enzyme form fHBP HA A | Sphingomonas paucimobilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.45 | (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O | i.e. 2'-hydroxybenzylidinepyruvate, i.e. 2'-hydroxybenzalpyruvate | Sphingomonas paucimobilis | salicylaldehyde + pyruvate | - |
? | |
| 4.1.2.45 | (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O | i.e. 2'-hydroxybenzylidinepyruvate, i.e. 2'-hydroxybenzalpyruvate | Sphingomonas paucimobilis TA-2 | salicylaldehyde + pyruvate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.45 | trimer | 3 * 37200, enzyme form fHBP HA B, SDS-PAGE | Sphingomonas paucimobilis |
| 4.1.2.45 | trimer | 3 * 37600, enzyme form fHBP HA A, SDS-PAGE | Sphingomonas paucimobilis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.45 | fHBP HA A | - |
Sphingomonas paucimobilis |
| 4.1.2.45 | fHBP HA B | - |
Sphingomonas paucimobilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 50 | - |
pH 7.3, 20 mM Tris-HCl buffer, 5 min, enzyme form fHBP HA A and enzyme form fHBP HA B, stable up to | Sphingomonas paucimobilis |
| 4.1.2.45 | 65 | - |
pH 7.3, 20 mM Tris-HCl buffer, 5 min, enzyme form fHBP HA A and enzyme form fHBP HA B, 80% loss of activity | Sphingomonas paucimobilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 9 | - |
enzyme form fHBP HA A and enzyme form fHBP HA B | Sphingomonas paucimobilis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 6 | 9.5 | pH 6: about 60% of maximal activity, pH 9.5: about 80% of maximal activity, enzyme form fHBP HA A and enzyme form fHBP HA B | Sphingomonas paucimobilis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.45 | 6 | 9.3 | 4°C, 20 h, enzyme form fHBP HA A is stable | Sphingomonas paucimobilis |
| 4.1.2.45 | 7.1 | 10.7 | 4°C, 20 h, enzyme form fHBP HA B is stable | Sphingomonas paucimobilis |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.1.2.45 | Sphingomonas paucimobilis | enzyme form fHBP HA B, isoelectric focusing | - |
4.3 |
| 4.1.2.45 | Sphingomonas paucimobilis | enzyme form fHBP HA A, isoelectric focusing | - |
4.8 |
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