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Literature summary extracted from

  • Kockelkorn, D.; Fuchs, G.
    Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: Enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales (2009), J. Bacteriol., 191, 6352-6362.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B47 expression in Escherichia coli Rosetta 2(DE3) Metallosphaera sedula
1.1.1.298 expression in Escherichia coli Rosetta 2(DE3) Metallosphaera sedula

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B47 additional information no inhibition by malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde. Incubation with 10 mM EDTA for 16 h or with 100 mM EDTA for 1 h at temperatures up to 65°C does not inactivate the enzyme, nor does addition of 10 mM EDTA to the assay mixture inhibit enzyme activity Metallosphaera sedula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B47 0.006
-
 NADPH 65°C, pH 7.9 Metallosphaera sedula
1.1.1.B47 0.052
-
 succinate semialdehyde 65°C, pH 7.9, cosubstrate: NADPH Metallosphaera sedula
1.1.1.B47 0.18
-
 NADH 65°C, pH 7.9 Metallosphaera sedula
1.1.1.298 0.07
-
 NADPH pH 7.4, 65°C Metallosphaera sedula
1.1.1.298 0.07
-
 malonate semialdehyde pH 7.4, 65°C Metallosphaera sedula

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.B47 additional information no stimulation by Mg2+ or Mn2+ (below 5 mM), or Fe2+ (below 1 mM) Metallosphaera sedula
1.1.1.B47 Zn2+ the enzyme contains 1.9 mol of zinc per mol of enzyme monomer Metallosphaera sedula
1.1.1.298 additional information addition of Zn2+ (0-0.04 mM) or Mg2+or Mn2+(0-5 mM) to the enzyme assay mixture neither stimulates nor inactivates the enzyme Metallosphaera sedula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.B47 40000
-
 2 * 40000, SDS-PAGE Metallosphaera sedula
1.1.1.B47 74000
-
 gel filtration Metallosphaera sedula
1.1.1.298 33000
-
 3 * 33000, SDS-PAGE Metallosphaera sedula
1.1.1.298 105000
-
 gel filtration Metallosphaera sedula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B47 succinate semialdehyde + NADPH + H+ Metallosphaera sedula the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea 4-hydroxybutanoate + NADP+
-
 ?
1.1.1.B47 succinate semialdehyde + NADPH + H+ Metallosphaera sedula DSM 5348 the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea 4-hydroxybutanoate + NADP+
-
 ?
1.1.1.298 malonate semialdehyde + NADPH + H+ Metallosphaera sedula the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea 3-hydroxypropanoate + NADP+
-
 ?
1.1.1.298 malonate semialdehyde + NADPH + H+ Metallosphaera sedula DSM 5348 the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea 3-hydroxypropanoate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B47 Metallosphaera sedula A4YGN0
-
-
1.1.1.B47 Metallosphaera sedula DSM 5348 A4YGN0
-
-
1.1.1.298 Metallosphaera sedula A4YI81
-
-
1.1.1.298 Metallosphaera sedula DSM 5348 A4YI81
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B47
-
 Metallosphaera sedula
1.1.1.298
-
 Metallosphaera sedula

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.B47 culture condition:autotrophically-grown cell no activity in heterotrophically-grown cell Metallosphaera sedula
-
1.1.1.298 culture condition:autotrophically-grown cell low activity in heterotrophically-grown cell Metallosphaera sedula
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B47 0.15
-
 cell extract, NADH-dependent reaction, 65°C, pH 7.9 Metallosphaera sedula
1.1.1.B47 1.4
-
 cell extract, NADPH-dependent reaction, 65°C, pH 7.9 Metallosphaera sedula
1.1.1.B47 700
-
 purified enzyme, NADPH-dependent reaction, 65°C, pH 7.9 Metallosphaera sedula
1.1.1.298 200
-
 pH 7.4, 65°C, heterologously expressed enzyme Metallosphaera sedula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B47 additional information malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate Metallosphaera sedula ?
-
 ?
1.1.1.B47 additional information malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate Metallosphaera sedula DSM 5348 ?
-
 ?
1.1.1.B47 succinate semialdehyde + NADH + H+ very low activity with NADH Metallosphaera sedula 4-hydroxybutanoate + NAD+
-
 ?
1.1.1.B47 succinate semialdehyde + NADH + H+ very low activity with NADH Metallosphaera sedula DSM 5348 4-hydroxybutanoate + NAD+
-
 ?
1.1.1.B47 succinate semialdehyde + NADPH + H+
-
 Metallosphaera sedula 4-hydroxybutanoate + NADP+
-
 ?
1.1.1.B47 succinate semialdehyde + NADPH + H+ the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula 4-hydroxybutanoate + NADP+
-
 ?
1.1.1.B47 succinate semialdehyde + NADPH + H+
-
 Metallosphaera sedula DSM 5348 4-hydroxybutanoate + NADP+
-
 ?
1.1.1.B47 succinate semialdehyde + NADPH + H+ the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula DSM 5348 4-hydroxybutanoate + NADP+
-
 ?
1.1.1.298 malonate semialdehyde + NADH + H+ NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula 3-hydroxypropanoate + NAD+
-
 ?
1.1.1.298 malonate semialdehyde + NADH + H+ NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula DSM 5348 3-hydroxypropanoate + NAD+
-
 ?
1.1.1.298 malonate semialdehyde + NADPH + H+ the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula 3-hydroxypropanoate + NADP+
-
 ?
1.1.1.298 malonate semialdehyde + NADPH + H+ NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula 3-hydroxypropanoate + NADP+
-
 ?
1.1.1.298 malonate semialdehyde + NADPH + H+ the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula DSM 5348 3-hydroxypropanoate + NADP+
-
 ?
1.1.1.298 malonate semialdehyde + NADPH + H+ NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula DSM 5348 3-hydroxypropanoate + NADP+
-
 ?
1.1.1.298 additional information succinic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate Metallosphaera sedula ?
-
 ?
1.1.1.298 additional information succinic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate Metallosphaera sedula DSM 5348 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.B47 homodimer 2 * 40000, SDS-PAGE Metallosphaera sedula
1.1.1.298 homotrimer 3 * 33000, SDS-PAGE Metallosphaera sedula

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B47 Msed_1424 locus name Metallosphaera sedula
1.1.1.B47 succinate semialdehyde reductase
-
 Metallosphaera sedula
1.1.1.B47 succinic semialdehyde reductase
-
 Metallosphaera sedula
1.1.1.B47 succinic semialdehyde reductase (NADPH)
-
 Metallosphaera sedula
1.1.1.298 malonic semialdehyde reductase
-
 Metallosphaera sedula
1.1.1.298 Msed_1993 locus name Metallosphaera sedula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B47 90
-
-
 Metallosphaera sedula
1.1.1.298 65
-
 assay at Metallosphaera sedula
1.1.1.298 75
-
-
 Metallosphaera sedula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.B47 440
-
 NADPH 65°C, pH 7.9 Metallosphaera sedula
1.1.1.B47 440
-
 succinate semialdehyde 65°C, pH 7.9 Metallosphaera sedula
1.1.1.298 115
-
 NADPH pH 7.4, 65°C Metallosphaera sedula
1.1.1.298 115
-
 malonate semialdehyde pH 7.4, 65°C Metallosphaera sedula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B47 7
-
 at 20°C Metallosphaera sedula
1.1.1.B47 7.5
-
 at 65°C Metallosphaera sedula
1.1.1.298 6.7
-
 optimum pH at 65°C Metallosphaera sedula
1.1.1.298 7.4
-
 asay at Metallosphaera sedula

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.B47 6.5 8 at 65°C, half-maximal activity at pH 6.5 and at pH 8.0 Metallosphaera sedula
1.1.1.B47 6.9 8.4 at 20°C, half-maximal activity at pH 6.9 and at pH 8.4 Metallosphaera sedula

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B47 NADPH
-
 Metallosphaera sedula
1.1.1.298 NADH NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula
1.1.1.298 NADPH NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula

Expression

EC Number Organism Comment Expression
1.1.1.298 Metallosphaera sedula the specific activity of this conversion in autotrophically grown cells is 1.8 microM/min * mg protein, whereas in heterotrophically grown cells the specific activity is 0.35 microM/min * mg protein up
1.2.1.75 Metallosphaera sedula extracts of autotrophically grown cells exhibit an NADPH-dependent succinyl-CoA reductase activity of 200 nM/min*mg of soluble protein, whereas extracts of heterotrophically grown cells exhibit an activity of 10 nM/min*mg of protein up
1.2.1.76 Metallosphaera sedula extracts of autotrophically grown cells exhibit an NADPH-dependent succinyl-CoA reductase activity of 200 nM/min*mg of soluble protein, whereas extracts of heterotrophically grown cells exhibit an activity of 10 nM/min*mg of protein up

General Information

EC Number General Information Comment Organism
1.1.1.B47 metabolism the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula
1.1.1.298 metabolism the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula
1.2.1.75 metabolism the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula
1.2.1.76 metabolism the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula