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Literature summary extracted from

  • Denger, K.; Mayer, J.; Buhmann, M.; Weinitschke, S.; Smits, T.H.; Cook, A.M.
    Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase (2009), J. Bacteriol., 191, 5648-5656.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.15 63000
-
 SDS-PAGE Roseovarius nubinhibens
4.1.1.79 235000
-
 native protein with alpha6beta6-structure, determined by gel filtration Roseovarius nubinhibens
4.4.1.25 35000
-
 SDS-PAGE Roseovarius nubinhibens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.15 2-sulfoacetaldehyde + phosphate Roseovarius nubinhibens
-
 acetyl phosphate + sulfite
-
 ?
4.1.1.79 3-sulfopyruvate Roseovarius nubinhibens
-
 2-sulfoacetaldehyde + CO2
-
 ?
4.4.1.25 L-cysteate + H2O Roseovarius nubinhibens
-
 pyruvate + bisulfite + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.15 Roseovarius nubinhibens A3SR25
-
-
4.1.1.79 Roseovarius nubinhibens A3SN11 and A3SN10 subunit alpha and beta
-
4.4.1.25 Roseovarius nubinhibens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.15 by anion exchange chromatography Roseovarius nubinhibens
4.1.1.79 by anion exchange chromatography, hydrophobic interaction chromatography and gel filtration Roseovarius nubinhibens
4.4.1.25 by anion exchange chromatography Roseovarius nubinhibens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.15 cell culture ISM strain Roseovarius nubinhibens
-
4.1.1.79 cell culture ISM strain Roseovarius nubinhibens
-
4.4.1.25 cell culture ISM strain Roseovarius nubinhibens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.15 additional information
-
 specific Xsc enzyme activity in extracts from cells grown with cysteate is 0.1 mkat/mg protein Roseovarius nubinhibens
2.3.3.15 additional information
-
 specific Xsc enzyme activity in extracts from cells grown with sulfolactate is 0.9 mkat/mg protein Roseovarius nubinhibens
2.3.3.15 additional information
-
 specific Xsc enzyme activity in extracts from cells grown with taurine is 1.5 mkat/mg protein Roseovarius nubinhibens
4.1.1.79 additional information
-
 specific ComDE enzyme activity in extracts from cells grown with sulfolactate is 0.9 mkat/mg protein, 0 mM Tris-HCl (pH 7.5), 1 mM thiamine diphosphate, 1 to 5 mM sulfopyruvate, and protein (0.1-1.0 mg/ml) Roseovarius nubinhibens
4.4.1.25 additional information
-
 specific CuyA enzyme activity in extracts from cells grown with cysteate is 5.2 mkat/mg protein Roseovarius nubinhibens
4.4.1.25 additional information
-
 specific CuyA enzyme activity in extracts from cells grown with sulfolactate is 1.0 mkat/mg protein Roseovarius nubinhibens

Storage Stability

EC Number Storage Stability Organism
4.1.1.79 purified ComDE is stable under an air atmosphere, it can be stored at 4°C for at least a month without loss of activity Roseovarius nubinhibens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.15 2-sulfoacetaldehyde + phosphate
-
 Roseovarius nubinhibens acetyl phosphate + sulfite
-
 ?
4.1.1.79 3-sulfopyruvate
-
 Roseovarius nubinhibens 2-sulfoacetaldehyde + CO2
-
 ?
4.4.1.25 L-cysteate + H2O
-
 Roseovarius nubinhibens pyruvate + bisulfite + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.79 heterodimer 2 subunits, determined by SDS-PAGE Roseovarius nubinhibens

Synonyms

EC Number Synonyms Comment Organism
2.3.3.15 sulfoacetaldehyde acetyltransferase
-
 Roseovarius nubinhibens
2.3.3.15 Xsc
-
 Roseovarius nubinhibens
4.1.1.79 3-sulfopyruvate decarboxylase
-
 Roseovarius nubinhibens
4.1.1.79 ComDE
-
 Roseovarius nubinhibens
4.4.1.25 CuyA
-
 Roseovarius nubinhibens
4.4.1.25 cysteate sulfo-lyase
-
 Roseovarius nubinhibens

General Information

EC Number General Information Comment Organism
2.3.3.15 physiological function XsC is involved in sulfonate degradation Roseovarius nubinhibens
4.1.1.79 physiological function ComDE is involved in sulfonate degradation Roseovarius nubinhibens
4.4.1.25 physiological function CuyA is involved in sulfonate degradation Roseovarius nubinhibens