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Literature summary extracted from

  • Bensing, B.A.; Sullam, P.M.
    Characterization of Streptococcus gordonii SecA2 as a paralogue of SecA (2009), J. Bacteriol., 191, 3482-3491.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.4.2.5 subcloned in Escherichia coli Streptococcus gordonii

Protein Variants

EC Number Protein Variants Comment Organism
7.4.2.5 additional information analysis of the function of SecA via mutants and SecA-SecA2-chimeras Streptococcus gordonii
7.4.2.5 additional information analysis of the function of SecA2 via mutants and SecA-SecA2-chimeras Streptococcus gordonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.4.2.5 azide 1.5 mM azide decrease the transport of the cell-surface glycoprotein mutant GspB736flag, 15 mM nearly prevent transport of GspB736flag, azide have no effect on ATP hydrolysis activity of the recombinant protein 6His-SecA; 1.5 mM azide markedly decrease the transport of the cell-surface glycoprotein mutant GspB736flag, 15 mM nearly prevent transport of GspB736flag, azide have no effect on ATP hydrolysis activity of the recombinant protein 6His-SecA2 Streptococcus gordonii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.4.2.5 membrane
-
 Streptococcus gordonii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.4.2.5 Mg2+ ATP hydrolysis activity of the recombinant protein 6His-SecA is highest with 0.1 mM Mg2+ and decreases both with higher and lower concentration of Mg2+ Streptococcus gordonii
7.4.2.5 Mg2+ ATP hydrolysis activity of the recombinant protein 6His-SecA2 aquires at least 0.5 mM Mg2+ and is higher with 2.5 mM Mg2+ Streptococcus gordonii

Organism

EC Number Organism UniProt Comment Textmining
7.4.2.5 Streptococcus gordonii Q9AET4 SecA
-
7.4.2.5 Streptococcus gordonii Q9AET6 SecA2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.4.2.5
-
 Streptococcus gordonii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.4.2.5 additional information
-
 circa 1.8 pMol Pi/pMol 6His-SecA2/min, measured in an assay containing 2.5 mM Mg2+ Streptococcus gordonii
7.4.2.5 additional information
-
 circa 7 pMol phosphate/pMol 6His-SecA/min measured in an assay containing 0.1 mM Mg2+ Streptococcus gordonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.5 ATP + H2O
-
 Streptococcus gordonii ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.4.2.5 SecA
-
 Streptococcus gordonii
7.4.2.5 SecA2 transporter system component specializing in the cell-surface glycoprotein GspB Streptococcus gordonii