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Literature summary extracted from

  • Yoon, S.H.; Moon, T.S.; Iranpour, P.; Lanza, A.M.; Prather, K.J.
    Cloning and characterization of uronate dehydrogenases from two pseudomonads and Agrobacterium tumefaciens strain C58 (2009), J. Bacteriol., 191, 1565-1573.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.203 udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC Pseudomonas syringae pv. tomato
1.1.1.203 udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC Agrobacterium tumefaciens
1.1.1.203 udh gene from genomic DNA amplified and inserted into pET21b containing a six-His tag and expressed in Escherichia coli BL21(DE3) or inserted into vector pTrc99SE and transformed into Escherichia coli mutant strain MG1655 DELTAuxaC Pseudomonas putida KT2440

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.203 0.04
-
 D-galacturonate
-
 Pseudomonas syringae pv. tomato
1.1.1.203 0.1
-
 D-galacturonate
-
 Pseudomonas putida KT2440
1.1.1.203 0.16
-
 D-galacturonate
-
 Agrobacterium tumefaciens
1.1.1.203 0.17
-
 NAD+
-
 Pseudomonas syringae pv. tomato
1.1.1.203 0.18
-
 NAD+
-
 Agrobacterium tumefaciens
1.1.1.203 0.21
-
 NAD+
-
 Pseudomonas putida KT2440
1.1.1.203 0.25
-
 D-glucuronate
-
 Pseudomonas putida KT2440
1.1.1.203 0.28
-
 D-glucuronate
-
 Pseudomonas syringae pv. tomato
1.1.1.203 0.37
-
 D-glucuronate
-
 Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.203 30000
-
 x * 30000, SDS-PAGE Pseudomonas syringae pv. tomato
1.1.1.203 30000
-
 x * 30000, SDS-PAGE Pseudomonas putida KT2440
1.1.1.203 32000
-
 x * 32000, SDS-PAGE Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.203 Agrobacterium tumefaciens Q7CRQ0
-
-
1.1.1.203 Agrobacterium tumefaciens C58 / ATCC 33970 Q7CRQ0
-
-
1.1.1.203 Pseudomonas putida KT2440 Q88NN6
-
-
1.1.1.203 Pseudomonas syringae pv. tomato Q888H1
-
-
1.1.1.203 Pseudomonas syringae pv. tomato DC3000 Q888H1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.203 gel filtration Pseudomonas syringae pv. tomato
1.1.1.203 gel filtration Agrobacterium tumefaciens
1.1.1.203 gel filtration Pseudomonas putida KT2440

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.203 D-galacturonate + NAD+ + H2O
-
 Pseudomonas syringae pv. tomato D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-galacturonate + NAD+ + H2O
-
 Agrobacterium tumefaciens D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-galacturonate + NAD+ + H2O
-
 Pseudomonas putida KT2440 D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-galacturonate + NAD+ + H2O
-
 Pseudomonas syringae pv. tomato DC3000 D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-galacturonate + NAD+ + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-galactarate + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ + H2O
-
 Pseudomonas syringae pv. tomato D-glucaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ + H2O
-
 Agrobacterium tumefaciens D-glucaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ + H2O
-
 Pseudomonas putida KT2440 D-glucaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ + H2O
-
 Pseudomonas syringae pv. tomato DC3000 D-glucaric acid + NADH + H+
-
 ?
1.1.1.203 D-glucuronate + NAD+ + H2O
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-glucaric acid + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.203 ? x * 32000, SDS-PAGE Agrobacterium tumefaciens
1.1.1.203 ? x * 30000, SDS-PAGE Pseudomonas syringae pv. tomato
1.1.1.203 ? x * 30000, SDS-PAGE Pseudomonas putida KT2440

Synonyms

EC Number Synonyms Comment Organism
1.1.1.203 Udh
-
 Pseudomonas syringae pv. tomato
1.1.1.203 Udh
-
 Agrobacterium tumefaciens
1.1.1.203 Udh
-
 Pseudomonas putida KT2440

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.203 0.24
-
 D-galacturonate
-
 Pseudomonas syringae pv. tomato
1.1.1.203 0.3
-
 D-galacturonate
-
 Pseudomonas putida KT2440
1.1.1.203 0.55
-
 D-glucuronate
-
 Pseudomonas putida KT2440
1.1.1.203 0.74
-
 D-glucuronate
-
 Pseudomonas syringae pv. tomato
1.1.1.203 0.92
-
 D-galacturonate
-
 Agrobacterium tumefaciens
1.1.1.203 1.9
-
 D-glucuronate
-
 Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.203 7
-
-
 Pseudomonas putida KT2440
1.1.1.203 8
-
-
 Pseudomonas syringae pv. tomato
1.1.1.203 8
-
-
 Agrobacterium tumefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.203 NAD+
-
 Pseudomonas syringae pv. tomato
1.1.1.203 NAD+
-
 Agrobacterium tumefaciens
1.1.1.203 NAD+
-
 Pseudomonas putida KT2440