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Literature summary extracted from

  • Baur, S.; Marles-Wright, J.; Buckenmaier, S.; Lewis, R.J.; Vollmer, W.
    Synthesis of CDP-activated ribitol for teichoic acid precursors in Streptococcus pneumoniae (2009), J. Bacteriol., 191, 1200-1210.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.405 spr1148 gene amplified, inserted into plasmid pJFK118EH and transformed into competent cells of Escherichia coli DH5alpha. Plasmid pJFK118EH1148 isolated and insert with the spr1148 gene cut off by restriction enzymes NdeI and SacI. Fragment ligated into the overexpression plasmid pET28a(+) to generate plasmid pET1148. The plasmid transformed into Escherichia coli BL21(DE3) for overexpression of spr1148 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.405 0.061
-
 D-ribulose 5-phosphate
-
 Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.405 Streptococcus pneumoniae Q8DPI3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.405 by gel filtration Streptococcus pneumoniae

Storage Stability

EC Number Storage Stability Organism
1.1.1.405 -20°C Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.405 D-ribulose 5-phosphate + NADPH + H+
-
 Streptococcus pneumoniae D-ribitol 5-phosphate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.405 TarJ
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.405 0.5
-
 D-ribulose 5-phosphate
-
 Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.405 additional information NADH is not consumed in the presence of Spr1148-His and ribulose 5-phosphate Streptococcus pneumoniae
1.1.1.405 NADPH
-
 Streptococcus pneumoniae