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Literature summary extracted from
- Characterization of abn2 (yxiA), encoding a Bacillus subtilis GH43 arabinanase, Abn2, and its role in arabino-polysaccharide degradation (2008), J. Bacteriol., 190, 4272-4280.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.99 | additional information | expression of abn2 is stimulated by arabinan and pectin, arabinose is not the natural inducer. EDTA does not affect the activity | Bacillus subtilis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.99 | Abn2 overexpressed in Escherichia coli BL21(DE3) pLysS harboring pZI39 | Bacillus subtilis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.99 | additional information | mutant strains IQB413 (DELTAabnA), IQB485 (DELTAabn2), and IQB486 (DELTAabnA DELTAabn2). Almost complete loss of activity in the DELTAabn2 DELTAabnA double mutant | Bacillus subtilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.99 | additional information | expression of abn2 is repressed by glucose. Glucose causes a 17- and 44fold repression of the abn2'-lacZ fusion expression during the exponential growth phase and the early postexponential phase, respectively | Bacillus subtilis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 52380 | - |
sequence analysis | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.99 | Bacillus subtilis | P42293 | - |
- |
| 3.2.1.99 | Bacillus subtilis 168T+ | P42293 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.99 | by gel filtration, more than 95% pure | Bacillus subtilis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.99 | -80°C, 20 mM Na-phosphate buffer, pH 7.4, 50 mM NaCl, 10% glycerol | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.99 | 1,5-alpha-L-arabinan + H2O | Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters | Bacillus subtilis | ? | - |
? |  |
| 3.2.1.99 | 1,5-alpha-L-arabinan + H2O | Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters | Bacillus subtilis 168T+ | ? | - |
? | |
| 3.2.1.99 | apple pectin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| 3.2.1.99 | apple pectin + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
| 3.2.1.99 | additional information | shows no activity toward larchwood arabinogalactan, wheat arabinoxylan, and p-nitrophenyl-alpha-L-arabinofuranoside | Bacillus subtilis | ? | - |
? | |
| 3.2.1.99 | additional information | shows no activity toward larchwood arabinogalactan, wheat arabinoxylan, and p-nitrophenyl-alpha-L-arabinofuranoside | Bacillus subtilis 168T+ | ? | - |
? | |
| 3.2.1.99 | red debranched arabinan + H2O | - |
Bacillus subtilis | ? | - |
? | |
| 3.2.1.99 | red debranched arabinan + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
| 3.2.1.99 | sugar beet arabinan + H2O | - |
Bacillus subtilis | ? | - |
? | |
| 3.2.1.99 | sugar beet arabinan + H2O | - |
Bacillus subtilis 168T+ | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.99 | Abn2 | - |
Bacillus subtilis |
| 3.2.1.99 | endo-alpha-1,5-L-arabinanase | - |
Bacillus subtilis |
| 3.2.1.99 | GH43 arabinanase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 50 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 50 | - |
remains fully active after 30 min of preincubation at 50°C. After preincubation at 60°C, the residual activity is only 15% | Bacillus subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.99 | 7 | - |
- |
Bacillus subtilis |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.99 | Bacillus subtilis | - |
- |
7.37 |
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