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Literature summary extracted from
- Cel9D, an atypical 1,4-beta-D-glucan glucohydrolase from Fibrobacter succinogenes: characteristics, catalytic residues, and synergistic interactions with other cellulases (2008), J. Bacteriol., 190, 1976-1984.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.74 | additional information | calcium ions are not required for its function | Fibrobacter succinogenes subsp. succinogenes S85 |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.74 | into vector pET30a and expressed in Escherichia coli BL21(DE3) | Fibrobacter succinogenes subsp. succinogenes S85 |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.74 | D166A | no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation | Fibrobacter succinogenes subsp. succinogenes S85 |
| 3.2.1.74 | DELTAS168 | no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation | Fibrobacter succinogenes subsp. succinogenes S85 |
| 3.2.1.74 | E612A | no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation | Fibrobacter succinogenes subsp. succinogenes S85 |
| 3.2.1.74 | S168A | retains about 40% of activity compared to the wild-type | Fibrobacter succinogenes subsp. succinogenes S85 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.74 | additional information | Cel9D has 27% lower activity on medium-viscosity carboxymethyl cellulose compared to the activity on low-viscosity carboxymethyl cellulose. Is not inhibited by 10 mM EDTA | Fibrobacter succinogenes subsp. succinogenes S85 |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.74 | 0.73 | - |
cellohexaose | - |
Fibrobacter succinogenes subsp. succinogenes S85 |  |
| 3.2.1.74 | 0.9 | - |
cellopentaose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
| 3.2.1.74 | 1.21 | - |
cellotetraose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
| 3.2.1.74 | 1.5 | 2 | cellotriose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
| 3.2.1.74 | 28 | - |
cellobiose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.74 | membrane | - |
Fibrobacter succinogenes subsp. succinogenes S85 | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 76000 | - |
1 * 76000, SDS-PAGE | Fibrobacter succinogenes subsp. succinogenes S85 |
| 3.2.1.74 | 77000 | - |
sequence analysis | Fibrobacter succinogenes subsp. succinogenes S85 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.74 | Fibrobacter succinogenes subsp. succinogenes S85 | B0FWI3 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.74 | wild-type and mutants purified by immobilized metal affinity chromatography. Wild-type further purified by gel filtration | Fibrobacter succinogenes subsp. succinogenes S85 |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 0.038 | - |
with avicel | Fibrobacter succinogenes subsp. succinogenes S85 |
| 3.2.1.74 | 0.25 | - |
with amorphous cellulose | Fibrobacter succinogenes subsp. succinogenes S85 |
| 3.2.1.74 | 0.275 | - |
with carboxymethyl cellulose | Fibrobacter succinogenes subsp. succinogenes S85 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.74 | acid swollen cellulose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | D-glucose + ? | - |
? | |
| 3.2.1.74 | amorphous cellulose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
| 3.2.1.74 | avicel + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
| 3.2.1.74 | carboxymethyl cellulose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
| 3.2.1.74 | cellobiose + H2O | is degraded very slowly | Fibrobacter succinogenes subsp. succinogenes S85 | 2 D-glucose | - |
? | |
| 3.2.1.74 | cellohexaose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | cellopentaose + D-glucose | - |
? | |
| 3.2.1.74 | cellopentaose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
| 3.2.1.74 | cellotetraose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
| 3.2.1.74 | cellotriose + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
| 3.2.1.74 | additional information | no activity on laminarin, sophorose, laminaribiose, gentiobiose, p-nitrophenol-alpha-L-arabinofuranoside, p-nitrophenol-alpha-D-glucoside, p-nitrophenol-beta-D-cellobioside, p-nitrophenol-beta-D-fucoside, p-nitrophenol-beta-D-galactoside, p-nitrophenol-N-acetyl-beta-D-glucosaminide, p-nitrophenol-beta-D-glucoside, p-nitrophenol-beta-D-glucuronoside, p-nitrophenol-beta-D-lactoside, or p-nitrophenol-beta-D-maltoside | Fibrobacter succinogenes subsp. succinogenes S85 | ? | - |
? | |
| 3.2.1.74 | p-nitrophenol beta-D-cellobioside + H2O | - |
Fibrobacter succinogenes subsp. succinogenes S85 | p-nitrophenol beta-D-glucopyranoside + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.74 | monomer | 1 * 76000, SDS-PAGE | Fibrobacter succinogenes subsp. succinogenes S85 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.74 | 1,4-beta-D-glucan glucohydrolase | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
| 3.2.1.74 | Cel9D | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
| 3.2.1.74 | family 9 glycoside hydrolase | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
| 3.2.1.74 | GH9 | - |
Fibrobacter succinogenes subsp. succinogenes S85 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 37 | - |
- |
Fibrobacter succinogenes subsp. succinogenes S85 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.74 | 0.25 | - |
cellobiose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
| 3.2.1.74 | 6.21 | - |
cellotriose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
| 3.2.1.74 | 16.3 | - |
cellohexaose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
| 3.2.1.74 | 16.4 | - |
cellopentaose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
| 3.2.1.74 | 18.6 | - |
cellotetraose | - |
Fibrobacter succinogenes subsp. succinogenes S85 | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 6.5 | - |
- |
Fibrobacter succinogenes subsp. succinogenes S85 |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.74 | 6 | 8 | retains 80% activity | Fibrobacter succinogenes subsp. succinogenes S85 |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.74 | Fibrobacter succinogenes subsp. succinogenes S85 | - |
- |
5.27 |
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