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Literature summary extracted from

  • Kneidinger, B.; Marolda, C.; Graninger, M.; Zamyatina, A.; McArthur, F.; Kosma, P.; Valvano, M.A.; Messner, P.
    Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli (2002), J. Bacteriol., 184, 363-369.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.82 overexpressed as His-tagged fusion protein Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.82 24900
-
x * 24900, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.167 D-glycero-D-manno-heptose 7-phosphate + ATP Escherichia coli synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase) D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP
-
?
2.7.7.70 D-glycero-beta-D-manno-heptose 1-phosphate + ATP Escherichia coli synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase) ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
?
3.1.3.82 D-glycero-D-manno-heptose 1,7-bisphosphate + H2O Escherichia coli synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase) D-glycero-D-manno-heptose 1-phosphate + phosphate
-
?
5.3.1.28 D-sedoheptulose 7-phosphate Escherichia coli synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase) D-glycero-alpha,beta-D-manno-heptose 7-phosphate
-
?
5.3.1.28 D-sedoheptulose 7-phosphate Aneurinibacillus thermoaerophilus
-
D-glycero-D-manno-heptose 7-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.167 Escherichia coli P76658 bifunctional protein hldE includes: D-beta-D-heptose 7-phosphate kinase and D-beta-D-heptose 1-phosphate adenosyltransferase
-
2.7.7.70 Escherichia coli P76658 bifunctional protein hldE includes: D-beta-D-heptose 7-phosphate kinase and D-beta-D-heptose 1-phosphate adenosyltransferase
-
3.1.3.82 Escherichia coli P63228
-
-
5.3.1.28 Aneurinibacillus thermoaerophilus Q9AGY7
-
-
5.3.1.28 Escherichia coli P63224
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.82
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.167 D-glycero-D-manno-heptose 7-phosphate + ATP
-
Escherichia coli D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP
-
?
2.7.1.167 D-glycero-D-manno-heptose 7-phosphate + ATP synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase) Escherichia coli D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP
-
?
2.7.7.70 D-glycero-beta-D-manno-heptose 1-phosphate + ATP
-
Escherichia coli ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
?
2.7.7.70 D-glycero-beta-D-manno-heptose 1-phosphate + ATP synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase) Escherichia coli ADP-D-glycero-beta-D-manno-heptose + diphosphate
-
?
3.1.3.82 D-glycero-D-manno-heptose 1,7-bisphosphate + H2O
-
Escherichia coli D-glycero-D-manno-heptose 1-phosphate + phosphate
-
?
3.1.3.82 D-glycero-D-manno-heptose 1,7-bisphosphate + H2O synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase) Escherichia coli D-glycero-D-manno-heptose 1-phosphate + phosphate
-
?
5.3.1.28 D-sedoheptulose 7-phosphate
-
Escherichia coli D-glycero-alpha,beta-D-manno-heptose 7-phosphate
-
?
5.3.1.28 D-sedoheptulose 7-phosphate synthesis of ADP-D-beta-D-heptose in Escherichia coli requires three proteins, GmhA (sedoheptulose 7-phosphate isomerase), HldE (bifunctional D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase), and GmhB (D,D-heptose 1,7-bisphosphate phosphatase) Escherichia coli D-glycero-alpha,beta-D-manno-heptose 7-phosphate
-
?
5.3.1.28 D-sedoheptulose 7-phosphate
-
Aneurinibacillus thermoaerophilus D-glycero-D-manno-heptose 7-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.82 ? x * 24900, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.1.167 D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase bifunctional enzyme Escherichia coli
2.7.7.70 D-beta-D-heptose 7-phosphate kinase/D-beta-D-heptose 1-phosphate adenylyltransferase bifunctional enzyme Escherichia coli
5.3.1.28 sedoheptulose 7-phosphate isomerase
-
Aneurinibacillus thermoaerophilus

General Information

EC Number General Information Comment Organism
3.1.3.82 malfunction deletion of gmhB causes a partial defect in the synthesis of the lipopolysaccharide core, resulting in the formation of heptoseless and heptose-rich forms Escherichia coli