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Literature summary extracted from

  • Ensign, S.A.
    Hyman, M.R.; Arp, D.J.: In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper (1993), J. Bacteriol., 175, 1971-1980.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.39 Serum albumin stimulates in vitro Nitrosomonas europaea
1.14.99.39 spermine stimulates in vitro Nitrosomonas europaea

General Stability

EC Number General Stability Organism
1.14.99.39 loss of AMO activity upon lysis of Nitrosomonas europaea results from the loss of copper from the enzyme, generating a catalytically inactive, yet stable and activable, form of the enzyme Nitrosomonas europaea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.39 Acetylene two populations of AMO in cell extracts. The low, copper-independent (residual) AMO activity is completely inactivated by acetylene in the absence of exogenously added copper. The copper-dependent (activable) AMO activity is protected against acetylene inactivation in the absence of copper. In the presence of copper both populations of AMO are inactivated by acetylene Nitrosomonas europaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.39 Cu2+ the addition of CuCl2 to cell extracts results in 5- to 15-fold stimulation of ammonia-dependent O2 consumption, ammonia-dependent nitrite production, and hydrazine-dependent ethane oxidation. Two populations of AMO in cell extracts. The low, copper-independent (residual) AMO activity is completely inactivated by acetylene in the absence of exogenously added copper. The copper-dependent (activable) AMO activity is protected against acetylene inactivation in the absence of copper. However, in the presence of copper both populations of AMO are inactivated by acetylene Nitrosomonas europaea
1.14.99.39 MgCl2 stimulates in vitro. Loss of enzyme activity upon lysis of Nitrosomonas europaea results from the loss of copper from the enzyme, generating a catalytically inactive, yet stable and activable, form of the enzyme Nitrosomonas europaea
1.14.99.39 additional information Zn2+, Co2+, Ni2+, Fe2+, Fe3+, Ca2+, Mg2+, Mn2+, Cr3+, and Ag+, are ineffective at stimulating AMO activity Nitrosomonas europaea

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.39 Nitrosomonas europaea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.39 ammonia + AH2 + O2
-
 Nitrosomonas europaea NH2OH + A + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.39 AMO
-
 Nitrosomonas europaea