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Literature summary extracted from

  • Britt, A.J.; Bruce, N.C.; Lowe, C.R.
    Identification of a cocaine esterase in a strain of Pseudomonas maltophilia (1992), J. Bacteriol., 174, 2087-2094.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.84 additional information no inhibition by 1 mM eserine or 1 mM p-hydroxymercuribenzoate. Cocaine esterase shows product inhibition with neither benzoate nor ecgonine methyl ester Stenotrophomonas maltophilia
3.1.1.84 phenylmethylsulfonyl fluoride 1 mM, complete inhibition Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.84 0.36
-
 cocaine pH 7.0, 30°C Stenotrophomonas maltophilia
3.1.1.84 1.75
-
 ethyl 2-hydroxybenzoate pH 7.0, 30°C Stenotrophomonas maltophilia
3.1.1.84 1.89
-
 ethyl benzoate pH 7.0, 30°C Stenotrophomonas maltophilia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.84 110000
-
 in the presence of the solubilizing agent cholate, gel filtration Stenotrophomonas maltophilia
3.1.1.84 129000
-
 1 * 129000, SDS-PAGE Stenotrophomonas maltophilia
3.1.1.84 410000
-
 in the absence of cholate, cocaine esterase has a native MW of 410000 and probably existed as a tetramer, gel filtration Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.84 cocaine + H2O Stenotrophomonas maltophilia strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O Stenotrophomonas maltophilia MB11L strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source ecgonine methyl ester + benzoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.84 Stenotrophomonas maltophilia
-
-
-
3.1.1.84 Stenotrophomonas maltophilia MB11L
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.84 partial Stenotrophomonas maltophilia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.84 3.5
-
-
 Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.84 cocaine + H2O
-
 Stenotrophomonas maltophilia ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source Stenotrophomonas maltophilia ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O
-
 Stenotrophomonas maltophilia MB11L ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 cocaine + H2O strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source Stenotrophomonas maltophilia MB11L ecgonine methyl ester + benzoate
-
 ?
3.1.1.84 ethyl 2-hydroxybenzoate + H2O
-
 Stenotrophomonas maltophilia ethanol + 2-hydroxybenzoate
-
 ?
3.1.1.84 ethyl 2-hydroxybenzoate + H2O
-
 Stenotrophomonas maltophilia MB11L ethanol + 2-hydroxybenzoate
-
 ?
3.1.1.84 ethyl benzoate + H2O
-
 Stenotrophomonas maltophilia ethanol + benzoate
-
 ?
3.1.1.84 ethyl benzoate + H2O
-
 Stenotrophomonas maltophilia MB11L ethanol + benzoate
-
 ?
3.1.1.84 additional information no activity with: atropine, ethyl 3-hydroxybenzoate or ethyl 4-hydroxybenzoate Stenotrophomonas maltophilia ?
-
 ?
3.1.1.84 additional information no activity with: atropine, ethyl 3-hydroxybenzoate or ethyl 4-hydroxybenzoate Stenotrophomonas maltophilia MB11L ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.84 monomer 1 * 129000, SDS-PAGE Stenotrophomonas maltophilia
3.1.1.84 tetramer in the absence of cholate, cocaine esterase has a native MW of 410000 and probably existed as a tetramer Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.84 30
-
 assay at Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.84 8
-
-
 Stenotrophomonas maltophilia