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Literature summary extracted from

  • Wysocka-Kapcinska, M.; Lutyk-Nadolska, J.; Kiliszek, M.; Plochocka, D.; Maciag, M.; Leszczynska, A.; Rytka, J.; Burzynska, B.
    Functional expression of human HMG-CoA reductase in Saccharomyces cerevisiae: a system to analyse normal and mutated versions of the enzyme in the context of statin treatment (2009), J. Appl. Microbiol., 106, 895-902.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.34 drug development a yeast expression system can serve to study the influence of selected mutations in human HMG-CoA reductase on the sensitivity of the enzyme to commonly prescribed statins, thus this model system is suitable for the development and selection of lipid-lowering drugs as well as for the examination of DNA sequence variations in the context of statin therapy Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.34 cDNA encoding the wild-type and mutant forms of human HMG-CoA reductase expressed under control of the yeast MET25 promoter in a Saccharomyces cerevisiae strain with deletions of both HMG1 and HMG2 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.34 additional information expression of human HMG-CoA reductase in yeast complements the lethal phenotype of Saccharomyces cerevisiae strains lacking the HMG1 and HMG2 genes Homo sapiens
1.1.1.34 Q766H restores viability of Saccharomyces cerevisiae strains lacking the HMG1 and HMG2 genes, thus is catalytically active in yeast cells. Q766H mutation, which affects the structure of the catalytic domain, increases the sensitivity of the enzyme towards statin treatment Homo sapiens
1.1.1.34 R393Q restores viability of Saccharomyces cerevisiae strains lacking the HMG1 and HMG2 genes, thus is catalytically active in yeast cells. R393Q mutation does not change the properties of the enzyme towards statin treatment Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.34 atorvastatin has a differentiating effect on wild-type and mutant forms of the human protein Homo sapiens
1.1.1.34 fluvastatin has a differentiating effect on wild-type and mutant forms of the human protein Homo sapiens
1.1.1.34 simvastatin does not allow differentiation between the wild-type and mutant forms of the human protein Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.34 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.34 blood samples of patients with cardiovascular disorders Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
1.1.1.34 HMG-CoA reductase
-
 Homo sapiens
1.1.1.34 HMG1
-
 Homo sapiens
1.1.1.34 HMG2
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.34 NADP+
-
 Homo sapiens