Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bao, Y.; Xie, H.; Shan, J.; Jiang, R.; Zhang, Y.; Guo, L.; Zhang, R.; Li, Y.
    Biochemical characteristics and function of a threonine dehydrogenase encoded by ste11 in Ebosin biosynthesis of Streptomyces sp. 139 (2009), J. Appl. Microbiol., 106, 1140-1146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.103 into pET-30a digested with KpnI–EcoRI to construct pET-ste11 and expressed in Escherichia coli BL21 Streptomyces sp. 139

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.103 additional information ste11 gene knocked out with a double crossover via homologous recombination. Monosaccharide composition of exopolysaccharide produced by the mutant strain is altered from that of ebosin. Bioactivity of the mutant is lower than ebosin Streptomyces sp. 139

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Streptomyces sp. 139
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103 by sonication, centrifugation and on a Ni-NTA resin column Streptomyces sp. 139

Storage Stability

EC Number Storage Stability Organism
1.1.1.103 -20°C Streptomyces sp. 139

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 L-threonine + NAD+
-
 Streptomyces sp. 139 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 additional information ste11 gene encoding a TDH may function as a modifier gene of ebosin during its biosynthesis Streptomyces sp. 139 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.103 TDH
-
 Streptomyces sp. 139
1.1.1.103 threonine dehydrogenase
-
 Streptomyces sp. 139

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.103 45
-
-
 Streptomyces sp. 139

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.103 25 45 enzyme shows 50% activity at 25°C compared with that at 35°C Streptomyces sp. 139

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.103 7.5
-
-
 Streptomyces sp. 139

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.103 5.5 7.5 activity at pH 5.5 is 50% of that at pH 7.5 Streptomyces sp. 139