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Literature summary extracted from

  • Chernykh, A.; Myasoedova, N.; Kolomytseva, M.; Ferraroni, M.; Briganti, F.; Scozzafava, A.; Golovleva, L.
    Laccase isoforms with unusual properties from the basidiomycete Steccherinum ochraceum strain 1833 (2008), J. Appl. Microbiol., 105, 2065-2075.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular high activity Steccherinum ochraceum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ a copper enzyme with type I Cu2+ Steccherinum ochraceum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 57000
-
x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE Steccherinum ochraceum
1.10.3.2 59000
-
x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE Steccherinum ochraceum
1.10.3.2 63000
-
x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE Steccherinum ochraceum

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Steccherinum ochraceum
-
three laccase isoforms: laccases I, II and III
-
1.10.3.2 Steccherinum ochraceum 1833
-
three laccase isoforms: laccases I, II and III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native isozymes about 50fold by anion exchange chromatography and gel filtration Steccherinum ochraceum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 19
-
purified isozyme laccase I Steccherinum ochraceum
1.10.3.2 20.5
-
purified isozyme laccase III Steccherinum ochraceum
1.10.3.2 22.3
-
purified isozyme laccase II Steccherinum ochraceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Steccherinum ochraceum ?
-
?
1.10.3.2 2,4-dimethylphenol + O2
-
Steccherinum ochraceum ?
-
?
1.10.3.2 2,6-dimethoxyphenol + O2
-
Steccherinum ochraceum ?
-
?
1.10.3.2 2,6-dimethoxyphenol + O2
-
Steccherinum ochraceum 1833 ?
-
?
1.10.3.2 2,6-dimethylphenol + O2
-
Steccherinum ochraceum ?
-
?
1.10.3.2 2-methylphenol + O2 i.e. guaiacol Steccherinum ochraceum ?
-
?
1.10.3.2 catechol + O2
-
Steccherinum ochraceum ?
-
?
1.10.3.2 catechol + O2
-
Steccherinum ochraceum 1833 ?
-
?
1.10.3.2 coniferyl alcohol + O2
-
Steccherinum ochraceum ?
-
?
1.10.3.2 coniferyl alcohol + O2
-
Steccherinum ochraceum 1833 ?
-
?
1.10.3.2 additional information isozyme laccase II shows a higher activity and wider substrate specificity compared to the other isozymes, overview Steccherinum ochraceum ?
-
?
1.10.3.2 additional information isozyme laccase II shows a higher activity and wider substrate specificity compared to the other isozymes, overview Steccherinum ochraceum 1833 ?
-
?
1.10.3.2 syringaldazine + O2
-
Steccherinum ochraceum ?
-
?
1.10.3.2 syringaldazine + O2
-
Steccherinum ochraceum 1833 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE Steccherinum ochraceum

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 More the enzyme is a polyphenol oxidase belonging to the family of multi-copper oxidases Steccherinum ochraceum
1.10.3.2 p-diphenol dioxygen oxidoreductase
-
Steccherinum ochraceum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 70
-
isozyme laccase I Steccherinum ochraceum
1.10.3.2 75
-
isozyme laccase II Steccherinum ochraceum
1.10.3.2 80
-
isozyme laccase III Steccherinum ochraceum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 25 80
-
Steccherinum ochraceum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 60
-
purified isozymes, half-lives are 5-6 h, after 8 h 20% remaining activity for isozyme laccase I, 40% for laccase II and III Steccherinum ochraceum
1.10.3.2 70
-
purified isozymes, 8-15% remainng activity after 4 h, 50% remaining activity after 1.7 h Steccherinum ochraceum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 additional information
-
the pH optima of the isozymes with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid are in the range of pH 1.2-2.0 Steccherinum ochraceum
1.10.3.2 4 5 isozyme laccase I, dependent on the substrate Steccherinum ochraceum
1.10.3.2 4.5 5 isozyme laccase II Steccherinum ochraceum
1.10.3.2 4.5 5 isozyme laccase III Steccherinum ochraceum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 6
-
Steccherinum ochraceum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 2 6 purified isozymes, 25°C, 1 week, stable Steccherinum ochraceum