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Literature summary extracted from

  • Paul, S.; Chaudhuri, T. K.
    Chaperone mediated solubilization of 69-kDa recombinant maltodextrin glucosidase in Escherichia coli (2008), J. Appl. Microbiol., 104, 35-41.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 overexpression in Escherichia coli DH5 alpha Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 cytoplasm
-
 Escherichia coli 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 69000
-
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Escherichia coli P21517
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 additional information
-
 aggregation of maltodextrin glucosidase in the cytoplasm is partially prevented by the co-expression of GroEL and GroES, and using externally supplemented glycerol or lowering the culture temperature. Co-expression of GroEL and GroES or simultaneous presence of overexpressed GroEL, GroES and externally supplemented glycerol together results significant increase of the activity of maltodextrin glucosidase Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 p-nitrophenyl-alpha-D-maltoside + H2O
-
 Escherichia coli p-nitrophenol + alpha-D-maltose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 maltodextrin glucosidase
-
 Escherichia coli
3.2.1.20 MalZ
-
 Escherichia coli