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Literature summary extracted from

  • Kurita, O.
    Increase of acetate ester-hydrolyzing esterase activity in mixed cultures of Saccharomyces cerevisiae and Pichia anomala (2008), J. Appl. Microbiol., 104, 1051-1058.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.6 industry enhancement of sensory quality in alcoholic beverage through higher activity of Saccharomyces cerevisiae EAHase in mixed culture with Pichia anomala low respirating mutant Wickerhamomyces anomalus
3.1.1.6 industry enhancement of sensory quality in alcoholic beverage through higher activity of Saccharomyces cerevisiae EAHase in mixed culture with Pichia anomala mutant with respiration deficiency Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Saccharomyces cerevisiae
-
 Kyokai 701
-
3.1.1.6 Wickerhamomyces anomalus
-
 wild-type strain NBRC and EAL-6, a low-respiratory, petite mutant of strain NBRC
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.6 additional information
-
 mutant strain, 61% lower ethyl acetate-hydrolysing esterase (EAHase) activity than in wild-type strain Wickerhamomyces anomalus
3.1.1.6 additional information
-
 mutant strain, 68% lower isoamyl acetate-hydrolysing esterase (IAHase) activity than in wild-type strain Wickerhamomyces anomalus
3.1.1.6 0.000225
-
 mutant strain, ethyl acetate-hydrolysing esterase (EAHase) activity Wickerhamomyces anomalus
3.1.1.6 0.000236
-
 mutant strain, isoamyl acetate-hydrolysing esterase (IAHase) activity Wickerhamomyces anomalus
3.1.1.6 0.000342
-
 wild-type strain, ethyl acetate-hydrolysing esterase (EAHase) activity Wickerhamomyces anomalus
3.1.1.6 0.000347
-
 wild-type strain, isoamyl acetate-hydrolysing esterase (IAHase) activity Wickerhamomyces anomalus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 ethyl acetate + H2O 25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts Wickerhamomyces anomalus ethanol + acetate
-
 ?
3.1.1.6 isoamyl acetate + H2O 25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts Wickerhamomyces anomalus isoamyl alcohol + acetate
-
 ?
3.1.1.6 additional information isoamyl acetate and ethyl acetate-synthesizing esterase activities in mutant strain 100fold less than respective acetate ester-hydrolysing esterase activities Wickerhamomyces anomalus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.6 acetate ester-hydrolysing esterase
-
 Wickerhamomyces anomalus
3.1.1.6 acetate ester-hydrolysing esterase ethyl acetate- and isoamyl acetate-hydrolysing esterase activities Saccharomyces cerevisiae