EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.2.6 | expressed in Escherichia coli TG1 | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.2.6 | R276C | site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
3.5.2.6 | R276G | site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
3.5.2.6 | R276H | site-directed mutagenesis, investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
3.5.2.6 | R276N | site-directed mutagenesis, investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
3.5.2.6 | R276S | site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
3.5.2.6 | R276W | site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.2.6 | clavulanic acid | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.6 | 0.118 | - |
cefotaxime | R276G mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.133 | - |
cefotaxime | R276H mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.143 | - |
cefotaxime | R276S mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.153 | - |
cefotaxime | R276W mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.157 | - |
cefotaxime | CTX-M-1 wild type, 25°C | Escherichia coli | |
3.5.2.6 | 0.163 | - |
cefotaxime | R276N mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.163 | - |
cefalotin | R276N mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.206 | - |
cefalotin | R276W mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.207 | - |
cefalotin | R276G mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.22 | - |
cefotaxime | R276C mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.28 | - |
cefalotin | R276H mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.298 | - |
cefalotin | R276C mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.35 | - |
cefalotin | R276S mutant, 25°C | Escherichia coli | |
3.5.2.6 | 0.433 | - |
cefalotin | CTX-M-1 wild type, 25°C | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.2.6 | 28000 | - |
SDS-PAGE | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.2.6 | Escherichia coli | P28585 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.2.6 | CTX-M-1 wild-type and six derived mutants are purified by affinity chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.2.6 | cefalotin + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | cefotaxime + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
3.5.2.6 | nitrocefin + H2O | - |
Escherichia coli | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.2.6 | ? | x * 28000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.2.6 | CTX-M beta-lactamase | - |
Escherichia coli |
3.5.2.6 | CTX-M-1 | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.2.6 | 25 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.2.6 | 21 | - |
cefotaxime | R276G mutant, 25°C | Escherichia coli | |
3.5.2.6 | 44 | - |
cefotaxime | R276S mutant, 25°C | Escherichia coli | |
3.5.2.6 | 91 | - |
cefotaxime | R276C mutant, 25°C | Escherichia coli | |
3.5.2.6 | 92 | - |
cefotaxime | R276W mutant, 25°C | Escherichia coli | |
3.5.2.6 | 107 | - |
cefotaxime | R276N mutant, 25°C | Escherichia coli | |
3.5.2.6 | 120 | - |
cefotaxime | R276H mutant, 25°C | Escherichia coli | |
3.5.2.6 | 140 | - |
cefotaxime | CTX-M-1 wild type, 25°C | Escherichia coli | |
3.5.2.6 | 630 | - |
cefalotin | R276H mutant, 25°C | Escherichia coli | |
3.5.2.6 | 680 | - |
cefalotin | R276G mutant, 25°C | Escherichia coli | |
3.5.2.6 | 700 | - |
cefalotin | R276C mutant, 25°C | Escherichia coli | |
3.5.2.6 | 1180 | - |
cefalotin | R276W mutant, 25°C | Escherichia coli | |
3.5.2.6 | 1190 | - |
cefalotin | CTX-M-1 wild type, 25°C | Escherichia coli | |
3.5.2.6 | 1290 | - |
cefalotin | R276N mutant, 25°C | Escherichia coli | |
3.5.2.6 | 1800 | - |
cefalotin | R276S mutant, 25°C | Escherichia coli |