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Literature summary extracted from

  • Perez-Llarena, F.J.; Cartelle, M.; Mallo, S.; Beceiro, A.; Perez, A.; Villanueva, R.; Romero, A.; Bonnet, R.; Bou, G.
    Structure-function studies of arginine at position 276 in CTX-M beta -lactamases (2008), J. Antimicrob. Chemother., 61, 792-797.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli TG1 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 R276C site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
3.5.2.6 R276G site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
3.5.2.6 R276H site-directed mutagenesis, investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
3.5.2.6 R276N site-directed mutagenesis, investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
3.5.2.6 R276S site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli
3.5.2.6 R276W site-directed mutagenesis, the MICs of cefotaxime are clearly lower, clearly reduced catalytic efficiency towards cefotaxime. Investigation of the role of Arg276 in cefotaxime hydrolysis Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 clavulanic acid
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.118
-
cefotaxime R276G mutant, 25°C Escherichia coli
3.5.2.6 0.133
-
cefotaxime R276H mutant, 25°C Escherichia coli
3.5.2.6 0.143
-
cefotaxime R276S mutant, 25°C Escherichia coli
3.5.2.6 0.153
-
cefotaxime R276W mutant, 25°C Escherichia coli
3.5.2.6 0.157
-
cefotaxime CTX-M-1 wild type, 25°C Escherichia coli
3.5.2.6 0.163
-
cefotaxime R276N mutant, 25°C Escherichia coli
3.5.2.6 0.163
-
cefalotin R276N mutant, 25°C Escherichia coli
3.5.2.6 0.206
-
cefalotin R276W mutant, 25°C Escherichia coli
3.5.2.6 0.207
-
cefalotin R276G mutant, 25°C Escherichia coli
3.5.2.6 0.22
-
cefotaxime R276C mutant, 25°C Escherichia coli
3.5.2.6 0.28
-
cefalotin R276H mutant, 25°C Escherichia coli
3.5.2.6 0.298
-
cefalotin R276C mutant, 25°C Escherichia coli
3.5.2.6 0.35
-
cefalotin R276S mutant, 25°C Escherichia coli
3.5.2.6 0.433
-
cefalotin CTX-M-1 wild type, 25°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 28000
-
SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Escherichia coli P28585
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 CTX-M-1 wild-type and six derived mutants are purified by affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 cefalotin + H2O
-
Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 cefotaxime + H2O
-
Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 nitrocefin + H2O
-
Escherichia coli (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 ? x * 28000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 CTX-M beta-lactamase
-
Escherichia coli
3.5.2.6 CTX-M-1
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.6 25
-
assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 21
-
cefotaxime R276G mutant, 25°C Escherichia coli
3.5.2.6 44
-
cefotaxime R276S mutant, 25°C Escherichia coli
3.5.2.6 91
-
cefotaxime R276C mutant, 25°C Escherichia coli
3.5.2.6 92
-
cefotaxime R276W mutant, 25°C Escherichia coli
3.5.2.6 107
-
cefotaxime R276N mutant, 25°C Escherichia coli
3.5.2.6 120
-
cefotaxime R276H mutant, 25°C Escherichia coli
3.5.2.6 140
-
cefotaxime CTX-M-1 wild type, 25°C Escherichia coli
3.5.2.6 630
-
cefalotin R276H mutant, 25°C Escherichia coli
3.5.2.6 680
-
cefalotin R276G mutant, 25°C Escherichia coli
3.5.2.6 700
-
cefalotin R276C mutant, 25°C Escherichia coli
3.5.2.6 1180
-
cefalotin R276W mutant, 25°C Escherichia coli
3.5.2.6 1190
-
cefalotin CTX-M-1 wild type, 25°C Escherichia coli
3.5.2.6 1290
-
cefalotin R276N mutant, 25°C Escherichia coli
3.5.2.6 1800
-
cefalotin R276S mutant, 25°C Escherichia coli