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Literature summary extracted from

  • Reetz, M.T.; Wu, S.
    Laboratory evolution of robust and enantioselective Baeyer-Villiger monooxygenases for asymmetric catalysis (2009), J. Am. Chem. Soc., 131, 15424-15432.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.92 expressed in Escherichia coli Top10 cells Thermobifida fusca

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.92 P440F higher subtrate variability, temperature optimum at 50C with range from 45-56C Thermobifida fusca
1.14.13.92 P440H higher subtrate variability, temperature optimum at 50C with range from 45-56C Thermobifida fusca
1.14.13.92 P440I higher subtrate variability, temperature optimum at 50C with range from 45-56C Thermobifida fusca
1.14.13.92 P440L higher subtrate variability, temperature optimum at 50C with range from 45-56C Thermobifida fusca
1.14.13.92 P440N higher subtrate variability, temperature optimum at 50C with range from 45-56C Thermobifida fusca
1.14.13.92 P440T higher subtrate variability, temperature optimum at 50C with range from 45-56C Thermobifida fusca
1.14.13.92 P440Y higher subtrate variability, temperature optimum at 50C with range from 45-58C Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.92 Thermobifida fusca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.92
-
 Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.92 phenylacetone + NADPH + O2 reaction is performed in presence of 2 U secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus and isopropanol to recover NADPH Thermobifida fusca benzyl acetate + NADP+ + H2O
-
 ?
1.14.13.92 rac-2-ethylcyclohexanone + NADPH + O2 substrate is only accepted by mutants of phenylacetone monooxygenase, reaction is performed in presence of 2 U secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus and isopropanol to recover NADPH Thermobifida fusca benzyl acetate + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.92 Baeyer-Villiger monooxygenase
-
 Thermobifida fusca
1.14.13.92 BVMO
-
 Thermobifida fusca
1.14.13.92 PAMO
-
 Thermobifida fusca
1.14.13.92 phenylacetone monooxygenase
-
 Thermobifida fusca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.92 56
-
-
 Thermobifida fusca

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.13.92 45 58 at 58C 75% of enzyme activity remains, at 60C activity is nearly zero, at 45C enzyme is fully active Thermobifida fusca