Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sabat, J.; Stuehr, D.J.; Yeh, S.R.; Rousseau, D.L.
    Characterization of the proximal ligand in the P420 form of inducible nitric oxide synthase (2009), J. Am. Chem. Soc., 131, 12186-12192.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 the oxygenase domain of iNOS with a C-terminal His6-tag is expressed in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 carbon monoxide carbon monoxide down-regulates iNOS activity by reducing its expression level or by inhibiting its activity by converting it to an inactive P420 form, the presence of dithiothreitol, L-Arg, or H4B partially inhibits the iNOSP450 to iNOSP420 conversion, whereas the presence of both L-Arg and 5,6,7,8-tetrahydro-L-biopterin completely prevents the transition Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Rattus norvegicus Q06518
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 Ni-NTA column chromatography, gel filtration Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Rattus norvegicus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.39 inducible nitric oxide synthase
-
 Rattus norvegicus
1.14.13.39 iNOS
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin
-
 Rattus norvegicus
1.14.13.39 Calmodulin
-
 Rattus norvegicus
1.14.13.39 NADPH
-
 Rattus norvegicus