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Literature summary extracted from

  • Patel, A.K.; Singh, V.K.; Moir, A.J.; Jagannadham, M.V.
    Biochemical and spectroscopic characterization of morning glory peroxidase from an invasive and hallucinogenic plant weed Ipomoea carnea (2008), J. Agric. Food Chem., 56, 9236-9245.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.7 K2Cr2O7 108.8% relative activity at 10 mM Ipomoea carnea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.7 acetonitrile 88.0% residual activity at 60% (v/v) Ipomoea carnea
1.11.1.7 beta-mercaptoethanol
-
 Ipomoea carnea
1.11.1.7 butanol 81.0% residual activity at 30% (v/v) Ipomoea carnea
1.11.1.7 Ca2+ 61.8% relative activity at 10 mM Ipomoea carnea
1.11.1.7 Co2+ 51.9% relative activity at 10 mM Ipomoea carnea
1.11.1.7 Cs+ 86.3% relative activity at 10 mM Ipomoea carnea
1.11.1.7 Cu2+ 80.8% relative activity at 10 mM Ipomoea carnea
1.11.1.7 dioxane 82.0% residual activity at 50% (v/v) Ipomoea carnea
1.11.1.7 DMSO 76.0% residual activity at 30% (v/v) Ipomoea carnea
1.11.1.7 EDTA 87.3% relative activity at 10 mM Ipomoea carnea
1.11.1.7 ethanol 82.0% residual activity at 65% (v/v) Ipomoea carnea
1.11.1.7 glutathione
-
 Ipomoea carnea
1.11.1.7 guanidine hydrochloride 84.2% residual activity at 3 mM Ipomoea carnea
1.11.1.7 guanidine isothiocyanate 82.4% residual activity at 1.5 mM Ipomoea carnea
1.11.1.7 Hg2+ complete inhibition at 10 mM Ipomoea carnea
1.11.1.7 hydroxylamine
-
 Ipomoea carnea
1.11.1.7 Isopropanol 80.0% residual activity at 45% (v/v) Ipomoea carnea
1.11.1.7 K+ 92.6% relative activity at 10 mM Ipomoea carnea
1.11.1.7 L-cysteine
-
 Ipomoea carnea
1.11.1.7 methanol 85.0% residual activity at 70% (v/v) Ipomoea carnea
1.11.1.7 Mg2+ 83.5% relative activity at 10 mM Ipomoea carnea
1.11.1.7 Mn2+ 93.2% relative activity at 10 mM Ipomoea carnea
1.11.1.7 additional information inhibition is not observed with ethylmaleinimide and urea Ipomoea carnea
1.11.1.7 Na+ 94.8% relative activity at 10 mM Ipomoea carnea
1.11.1.7 Ni2+ 90.6% relative activity at 10 mM Ipomoea carnea
1.11.1.7 Pb2+ 91.8% relative activity at 10 mM Ipomoea carnea
1.11.1.7 SDS 81.7% residual activity at 10 mM Ipomoea carnea
1.11.1.7 Sodium azide
-
 Ipomoea carnea
1.11.1.7 Zn2+ 95.0% relative activity at 10 mM Ipomoea carnea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.12
-
 4-aminoantipyrine at pH 7.0 Ipomoea carnea
1.11.1.7 0.29
-
 pyrogallol at pH 5.5 Ipomoea carnea
1.11.1.7 0.5
-
 o-Dianisidine at pH 8.0 Ipomoea carnea
1.11.1.7 1.63
-
 pyrogallol at pH 6.0 Ipomoea carnea
1.11.1.7 2.02
-
 o-phenylenediamine at pH 5.5 Ipomoea carnea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.7 40000 45000 the purified enzyme migrates on SDS-PAGE as a single band with an apparent molecular mass of 40000-45000 Da Ipomoea carnea
1.11.1.7 42060
-
 MALDI-TOF mass spectrometry Ipomoea carnea

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Ipomoea carnea
-
 subspecies Ipomoea carnea fistulosa, morning glory
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.7 glycoprotein
-
 Ipomoea carnea

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, ether-Toyopearl column chromatography, and Sephacryl S-200 gel filtration Ipomoea carnea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 latex
-
 Ipomoea carnea
-
1.11.1.7 stem
-
 Ipomoea carnea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 4-aminoantipyrine + H2O2
-
 Ipomoea carnea ?
-
 ?
1.11.1.7 guaiacol + H2O2
-
 Ipomoea carnea tetraguaiacol + H2O
-
 ?
1.11.1.7 o-dianisidine + H2O2
-
 Ipomoea carnea ?
-
 ?
1.11.1.7 o-phenylenediamine + H2O2
-
 Ipomoea carnea ?
-
 ?
1.11.1.7 pyrogallol + H2O2
-
 Ipomoea carnea purpurogallin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 donor: H2O2 oxidoreductase
-
 Ipomoea carnea
1.11.1.7 MGP
-
 Ipomoea carnea

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.7 heme
-
 Ipomoea carnea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.7 Ipomoea carnea isoelectric focusing
-
 4.3