EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.8 | industry | high production yields of the modular xylanase Xyl30 by solid-state fermentation and its biochemical features make it a good candidate for use in industrial applications | Streptomyces avermitilis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.8 | 3588-bp BamHI-XbaI fragment containing genes xyl30 and hd30 introduced into the pN702GEM3 plasmid and overexpressed in Streptomyces lividans strain 66 | Streptomyces avermitilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | additional information | modular endoxylanase containing a xylan-binding domain, which after processing in the culture supernatant loses the afore mentioned domain and thus its capacity to bind xylan. Sharp decrease (up to 50%) in activity after 15 min of incubation at 70 or 80°C | Streptomyces avermitilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 35000 | - |
1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later | Streptomyces avermitilis |
3.2.1.8 | 42800 | - |
1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later | Streptomyces avermitilis |
3.2.1.8 | 47000 | - |
sequence analysis, xyl30 ORF | Streptomyces avermitilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Streptomyces avermitilis | Q9X584 | - |
- |
3.2.1.8 | Streptomyces avermitilis CECT 3339 | Q9X584 | - |
- |
3.2.1.8 | Streptomyces lividans | - |
- |
- |
3.2.1.8 | Streptomyces lividans 66 | - |
- |
- |
3.2.1.8 | Thermomyces lanuginosus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.8 | heavier protein band (42.8 kDa) partially purified | Streptomyces avermitilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | arabinoxylan + H2O | - |
Thermomyces lanuginosus | ? | - |
? | |
3.2.1.8 | arabinoxylan + H2O | highest production of xylanase activity after approximately 3 days of incubation. Only the heavier protein band (42.8 kDa) binds to the substrate | Streptomyces avermitilis | ? | - |
? | |
3.2.1.8 | arabinoxylan + H2O | highest production of xylanase activity after approximately 3 days of incubation. Only the heavier protein band (42.8 kDa) binds to the substrate | Streptomyces avermitilis CECT 3339 | ? | - |
? | |
3.2.1.8 | rye bran + H2O | low activity with Streptomyces lividans carrying empty vector pN702GEM3 | Streptomyces lividans | ? | - |
? | |
3.2.1.8 | rye bran + H2O | maximum production of xylanase occurs after 144 h of incubation. When waterwashed rye bran is used as substrate, maximum production is reached after 216 h | Streptomyces avermitilis | ? | - |
? | |
3.2.1.8 | rye bran + H2O | maximum production of xylanase occurs after 144 h of incubation. When waterwashed rye bran is used as substrate, maximum production is reached after 216 h | Streptomyces avermitilis CECT 3339 | ? | - |
? | |
3.2.1.8 | rye bran + H2O | low activity with Streptomyces lividans carrying empty vector pN702GEM3 | Streptomyces lividans 66 | ? | - |
? | |
3.2.1.8 | wheat bran + H2O | low activity with strain Streptomyces lividans carrying empty vector pN702GEM3 | Streptomyces lividans | ? | - |
? | |
3.2.1.8 | wheat bran + H2O | when waterwashed wheat bran is used as substrate, maximum production is reached after 216 h | Streptomyces avermitilis | ? | - |
? | |
3.2.1.8 | wheat bran + H2O | when waterwashed wheat bran is used as substrate, maximum production is reached after 216 h | Streptomyces avermitilis CECT 3339 | ? | - |
? | |
3.2.1.8 | wheat bran + H2O | low activity with strain Streptomyces lividans carrying empty vector pN702GEM3 | Streptomyces lividans 66 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.8 | ? | 1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later | Streptomyces avermitilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.8 | endoxylanase | - |
Streptomyces lividans |
3.2.1.8 | endoxylanase | - |
Thermomyces lanuginosus |
3.2.1.8 | endoxylanase | - |
Streptomyces avermitilis |
3.2.1.8 | Xyl30 | - |
Streptomyces avermitilis |
3.2.1.8 | xylanase | - |
Streptomyces lividans |
3.2.1.8 | xylanase | - |
Thermomyces lanuginosus |
3.2.1.8 | xylanase | - |
Streptomyces avermitilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 60 | - |
purified heavier protein band (42.8 kDa) | Streptomyces avermitilis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 20 | 80 | purified heavier protein band (42.8 kDa), more than 50% of maximum activity | Streptomyces avermitilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 70 | - |
purified heavier protein band (42.8 kDa), retains more than 40% after a 1 h incubation at 70°C. Retains 35% of its activity after a 1 h incubation at 87°C | Streptomyces avermitilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 7.5 | - |
purified heavier protein band (42.8 kDa) | Streptomyces avermitilis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 3 | 9 | purified heavier protein band (42.8 kDa), retains more than 50% of its activity | Streptomyces avermitilis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.8 | Streptomyces avermitilis | - |
- |
6.3 |