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Literature summary extracted from

  • Hernandez, A.; Lopez, J.C.; Santamaria, R.; Diaz, M.; Fernandez-Abalos, J.M.; Copa-Patino, J.L.; Soliveri, J.
    Xylan-binding xylanase Xyl30 from Streptomyces avermitilis: cloning, characterization, and overproduction in solid-state fermentation (2008), Int. Microbiol., 11, 133-141.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 industry high production yields of the modular xylanase Xyl30 by solid-state fermentation and its biochemical features make it a good candidate for use in industrial applications Streptomyces avermitilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 3588-bp BamHI-XbaI fragment containing genes xyl30 and hd30 introduced into the pN702GEM3 plasmid and overexpressed in Streptomyces lividans strain 66 Streptomyces avermitilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 additional information modular endoxylanase containing a xylan-binding domain, which after processing in the culture supernatant loses the afore mentioned domain and thus its capacity to bind xylan. Sharp decrease (up to 50%) in activity after 15 min of incubation at 70 or 80°C Streptomyces avermitilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 35000
-
1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later Streptomyces avermitilis
3.2.1.8 42800
-
1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later Streptomyces avermitilis
3.2.1.8 47000
-
sequence analysis, xyl30 ORF Streptomyces avermitilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Streptomyces avermitilis Q9X584
-
-
3.2.1.8 Streptomyces avermitilis CECT 3339 Q9X584
-
-
3.2.1.8 Streptomyces lividans
-
-
-
3.2.1.8 Streptomyces lividans 66
-
-
-
3.2.1.8 Thermomyces lanuginosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 heavier protein band (42.8 kDa) partially purified Streptomyces avermitilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 arabinoxylan + H2O
-
Thermomyces lanuginosus ?
-
?
3.2.1.8 arabinoxylan + H2O highest production of xylanase activity after approximately 3 days of incubation. Only the heavier protein band (42.8 kDa) binds to the substrate Streptomyces avermitilis ?
-
?
3.2.1.8 arabinoxylan + H2O highest production of xylanase activity after approximately 3 days of incubation. Only the heavier protein band (42.8 kDa) binds to the substrate Streptomyces avermitilis CECT 3339 ?
-
?
3.2.1.8 rye bran + H2O low activity with Streptomyces lividans carrying empty vector pN702GEM3 Streptomyces lividans ?
-
?
3.2.1.8 rye bran + H2O maximum production of xylanase occurs after 144 h of incubation. When waterwashed rye bran is used as substrate, maximum production is reached after 216 h Streptomyces avermitilis ?
-
?
3.2.1.8 rye bran + H2O maximum production of xylanase occurs after 144 h of incubation. When waterwashed rye bran is used as substrate, maximum production is reached after 216 h Streptomyces avermitilis CECT 3339 ?
-
?
3.2.1.8 rye bran + H2O low activity with Streptomyces lividans carrying empty vector pN702GEM3 Streptomyces lividans 66 ?
-
?
3.2.1.8 wheat bran + H2O low activity with strain Streptomyces lividans carrying empty vector pN702GEM3 Streptomyces lividans ?
-
?
3.2.1.8 wheat bran + H2O when waterwashed wheat bran is used as substrate, maximum production is reached after 216 h Streptomyces avermitilis ?
-
?
3.2.1.8 wheat bran + H2O when waterwashed wheat bran is used as substrate, maximum production is reached after 216 h Streptomyces avermitilis CECT 3339 ?
-
?
3.2.1.8 wheat bran + H2O low activity with strain Streptomyces lividans carrying empty vector pN702GEM3 Streptomyces lividans 66 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? 1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later Streptomyces avermitilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endoxylanase
-
Streptomyces lividans
3.2.1.8 endoxylanase
-
Thermomyces lanuginosus
3.2.1.8 endoxylanase
-
Streptomyces avermitilis
3.2.1.8 Xyl30
-
Streptomyces avermitilis
3.2.1.8 xylanase
-
Streptomyces lividans
3.2.1.8 xylanase
-
Thermomyces lanuginosus
3.2.1.8 xylanase
-
Streptomyces avermitilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
purified heavier protein band (42.8 kDa) Streptomyces avermitilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 20 80 purified heavier protein band (42.8 kDa), more than 50% of maximum activity Streptomyces avermitilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 70
-
purified heavier protein band (42.8 kDa), retains more than 40% after a 1 h incubation at 70°C. Retains 35% of its activity after a 1 h incubation at 87°C Streptomyces avermitilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 7.5
-
purified heavier protein band (42.8 kDa) Streptomyces avermitilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 3 9 purified heavier protein band (42.8 kDa), retains more than 50% of its activity Streptomyces avermitilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Streptomyces avermitilis
-
-
6.3