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Literature summary extracted from

  • Fitzpatrick, J.M.; Fuentes, J.M.; Chalmers, I.W.; Wynn, T.A.; Modolell, M.; Hoffmann, K.F.; Hesse, M.
    Schistosoma mansoni arginase shares functional similarities with human orthologs but depends upon disulphide bridges for enzymatic activity (2009), Int. J. Parasitol., 39, 267-279.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.1 expressed in Escherichia coli strain DH5alpha Schistosoma mansoni

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.1 L-cysteine strongly blocked SmARG-activity Schistosoma mansoni
3.5.3.1 L-histidine strongly blocked SmARG-activity Schistosoma mansoni
3.5.3.1 L-ornithine moderately inhibitory Schistosoma mansoni
3.5.3.1 additional information L-lysine and diaminopimelic acid failed to block SmARG activity Schistosoma mansoni
3.5.3.1 NOHA
-
 Schistosoma mansoni
3.5.3.1 Nomega-hydroxy-nor-L-arginine norNOHA Schistosoma mansoni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.1 17
-
 L-arginine pH 10, 37°C Schistosoma mansoni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.1 Mn2+
-
 Schistosoma mansoni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.1 40000
-
 6 * 40000, SDS-PAGE Schistosoma mansoni
3.5.3.1 240000
-
 gel filtration Schistosoma mansoni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.1 additional information Schistosoma mansoni infection of mice with Schistosoma mansoni cercariae elevates arginase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.1 Schistosoma mansoni Q6TA39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.1
-
 Schistosoma mansoni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.1 669
-
-
 Schistosoma mansoni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.1 L-arginine + H2O
-
 Schistosoma mansoni L-ornithine + urea
-
 ?
3.5.3.1 additional information infection of mice with Schistosoma mansoni cercariae elevates arginase activity Schistosoma mansoni ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.1 hexamer 6 * 40000, SDS-PAGE Schistosoma mansoni

Synonyms

EC Number Synonyms Comment Organism
3.5.3.1 arginase
-
 Schistosoma mansoni
3.5.3.1 SmARG
-
 Schistosoma mansoni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.1 37
-
 assay at Schistosoma mansoni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.1 537
-
 L-arginine pH 10, 37°C Schistosoma mansoni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.1 10
-
 assay at Schistosoma mansoni

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.3.1 1.9
-
 L-ornithine pH 10, 37°C Schistosoma mansoni