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Literature summary extracted from

  • Motta, L.S.; Ramos, I.B.; Gomes, F.M.; de Souza, W.; Champagne, D.E.; Santiago, M.F.; Docampo, R.; Miranda, K.; Machado, E.A.
    Proton-pyrophosphatase and polyphosphate in acidocalcisome-like vesicles from oocytes and eggs of Periplaneta americana (2009), Insect Biochem. Mol. Biol., 39, 198-206.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.3.1 Ca2+
-
 Periplaneta americana
7.1.3.1 Imidodiphosphate when diphosphate concentration in the medium is adjusted to 0.05 mM, the enzyme is totally inhibited by 0.5 mM imidodiphosphate, the same concentration of imidodiphosphate only decreases the enzyme activity by 70% and 50% when diphosphate concentration in the medium is increased to 0.15 mM and 0.3 mM, respectively Periplaneta americana
7.1.3.1 additional information not inhibited by K+ Periplaneta americana
7.1.3.1 Sodium fluoride
-
 Periplaneta americana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.3.1 acidocalcisome commonly present in membranes of acidocalcisomes Periplaneta americana 20022
-
7.1.3.1 vesicle preferentially located in the small vesicles Periplaneta americana 31982
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.3.1 Mg2+ dependent on, maximum activity is obtained in the presence of 5 mM Mg2+, no diphosphate hydrolysis is detected in the absence of Mg2+ Periplaneta americana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.3.1 65000
-
 SDS-PAGE Periplaneta americana

Organism

EC Number Organism UniProt Comment Textmining
7.1.3.1 Periplaneta americana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.3.1 egg
-
 Periplaneta americana
-
7.1.3.1 oocyte
-
 Periplaneta americana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.3.1 diphosphate + H2O + H+[side 1]
-
 Periplaneta americana 2 phosphate + H+[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.3.1 H+-PPase
-
 Periplaneta americana
7.1.3.1 proton-pyrophosphatase electrogenic proton-pump that couples diphosphate hydrolysis to the active transport of protons across membranes Periplaneta americana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.3.1 7
-
 activity optimum at pH around 7.0 Periplaneta americana