EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.4 | dithiothreitol | addition of 2 mM dithiothreitol greatly enhances the tryptic activity | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.4 | Bowman-Birk inhibitor | double-headed native trypsin/chymotrypsin inhibitor in soybeans, which inhibits trypsin and chymotrypsin in a non-competitive manner, 78% reduced activity in the presence of 0.0005 mM Bowman-Birk inhibitor | Drosophila melanogaster | |
3.4.21.4 | Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride | inhibits trypsin specifically and irreversibly, 40% reduced activity in the presence of 1 mM Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.4 | DrmSP8-36 + H2O | Drosophila melanogaster | trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.4 | Drosophila melanogaster | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.21.4 | hemolymph | - |
Drosophila melanogaster | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.4 | DrmSP8-36 + H2O | trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph | Drosophila melanogaster | ? | - |
? | |
3.4.21.4 | N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O | - |
Drosophila melanogaster | N-alpha-benzoyl-DL-Arg + 4-nitroaniline | - |
? | |
3.4.21.4 | N-alpha-benzoyl-L-Phe-L-Val-L-Arg-4-nitroanilide + H2O | - |
Drosophila melanogaster | N-alpha-benzoyl-L-Phe-L-Val-L-Arg + 4-nitroaniline | - |
? | |
3.4.21.4 | N-alpha-benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O | - |
Drosophila melanogaster | N-alpha-benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline | - |
? |