Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pilpel, N.; Nezer, I.; Applebaum, S.W.; Heifetz, Y.
    Mating-increases trypsin in female Drosophila hemolymph (2008), Insect Biochem. Mol. Biol., 38, 320-330.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.4 dithiothreitol addition of 2 mM dithiothreitol greatly enhances the tryptic activity Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 Bowman-Birk inhibitor double-headed native trypsin/chymotrypsin inhibitor in soybeans, which inhibits trypsin and chymotrypsin in a non-competitive manner, 78% reduced activity in the presence of 0.0005 mM Bowman-Birk inhibitor Drosophila melanogaster
3.4.21.4 Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride inhibits trypsin specifically and irreversibly, 40% reduced activity in the presence of 1 mM Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.4 DrmSP8-36 + H2O Drosophila melanogaster trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Drosophila melanogaster
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 hemolymph
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 DrmSP8-36 + H2O trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph Drosophila melanogaster ?
-
?
3.4.21.4 N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
-
Drosophila melanogaster N-alpha-benzoyl-DL-Arg + 4-nitroaniline
-
?
3.4.21.4 N-alpha-benzoyl-L-Phe-L-Val-L-Arg-4-nitroanilide + H2O
-
Drosophila melanogaster N-alpha-benzoyl-L-Phe-L-Val-L-Arg + 4-nitroaniline
-
?
3.4.21.4 N-alpha-benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
-
Drosophila melanogaster N-alpha-benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
-
?