Literature summary extracted from
Shaw, C.A.; Starnbach, M.N.
Both CD4+ and CD8+ T cells respond to antigens fused to anthrax lethal toxin (2008), Infect. Immun., 76, 2603-2611.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.24.83 |
molecular biology |
results suggest that this toxin delivery system is capable of stimulating protective immune responses where effective immunization requires stimulation of both classes of T cells |
Bacillus anthracis |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.24.83 |
recombinant anthrax lethal toxin proteins consisting a model CD4 T-cell epitope from chicken ovalbumin (Ova) fused to nontoxic lethal factor (LFn). The antigen tags are generated by annealing single-stranded DNA oligonucleotides to form double-stranded DNA Plasmids are transformed into Escherichia coli BL21 |
Bacillus anthracis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.24.83 |
additional information |
in order to examine the role of protective antigen a lethal factor (LFn) fusion protein bearing two epitopes from Ova, one restricted by MHC-II and one restricted by MHC-I is generated. This single LFn fusion protein is capable of stimulating both ovalbumin-specific CD4+ and ovalbumin-specific CD8+ T-cell responses in mice |
Bacillus anthracis |
3.4.24.83 |
additional information |
it is investigated whether lethal factor (LFn) fusion proteins and protective antigen can also be used to deliver antigen to the MHC-II pathway for the stimulation of antigen-specific CD4+ T-cells. A CD4+ T-cell epitope from chicken ovalbumin is fused to nontoxic LFn and demonstrates that this recombinant protein induces an ovalbumin-specific CD4+ T-cell response both in vitro and in mice |
Bacillus anthracis |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
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Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.24.83 |
Bacillus anthracis |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.24.83 |
the recombinant LFn fusion proteins contain a vector-encoded His6 tag at the amino terminus and are purified by Ni2+-affinity chromatography to greater than 95% purity as determined by Coomassie staining |
Bacillus anthracis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.24.83 |
anthrax lethal toxin |
- |
Bacillus anthracis |
3.4.24.83 |
lethal factor |
lethal toxin is secreted by Bacillus anthracis as a bipartite toxin consisting of protective antigen (PA) and lethal factor (LF) |
Bacillus anthracis |