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Literature summary extracted from

  • Shaw, C.A.; Starnbach, M.N.
    Both CD4+ and CD8+ T cells respond to antigens fused to anthrax lethal toxin (2008), Infect. Immun., 76, 2603-2611.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.24.83 molecular biology results suggest that this toxin delivery system is capable of stimulating protective immune responses where effective immunization requires stimulation of both classes of T cells Bacillus anthracis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.83 recombinant anthrax lethal toxin proteins consisting a model CD4 T-cell epitope from chicken ovalbumin (Ova) fused to nontoxic lethal factor (LFn). The antigen tags are generated by annealing single-stranded DNA oligonucleotides to form double-stranded DNA Plasmids are transformed into Escherichia coli BL21 Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.83 additional information in order to examine the role of protective antigen a lethal factor (LFn) fusion protein bearing two epitopes from Ova, one restricted by MHC-II and one restricted by MHC-I is generated. This single LFn fusion protein is capable of stimulating both ovalbumin-specific CD4+ and ovalbumin-specific CD8+ T-cell responses in mice Bacillus anthracis
3.4.24.83 additional information it is investigated whether lethal factor (LFn) fusion proteins and protective antigen can also be used to deliver antigen to the MHC-II pathway for the stimulation of antigen-specific CD4+ T-cells. A CD4+ T-cell epitope from chicken ovalbumin is fused to nontoxic LFn and demonstrates that this recombinant protein induces an ovalbumin-specific CD4+ T-cell response both in vitro and in mice Bacillus anthracis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.83 Bacillus anthracis
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-
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.83 the recombinant LFn fusion proteins contain a vector-encoded His6 tag at the amino terminus and are purified by Ni2+-affinity chromatography to greater than 95% purity as determined by Coomassie staining Bacillus anthracis

Synonyms

EC Number Synonyms Comment Organism
3.4.24.83 anthrax lethal toxin
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Bacillus anthracis
3.4.24.83 lethal factor lethal toxin is secreted by Bacillus anthracis as a bipartite toxin consisting of protective antigen (PA) and lethal factor (LF) Bacillus anthracis