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Literature summary extracted from

  • Chen, F.; Kakizaki, I.; Yamaguchi, M.; Kojima, K.; Takagaki, K.; Endo, M.
    Novel products in hyaluronan digested by bovine testicular hyaluronidase (2008), Glycoconj. J., 26, 559-566.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.35 biotechnology novel products in hyaluronan digested by commercially available bovine testicular hyaluronidase are found which originate from a novel enzyme contaminat in the BTH Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 testis
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 chondroitin + H2O
-
 Bos taurus ?
-
 ?
3.2.1.35 chondroitin 4-sulfate + H2O
-
 Bos taurus ?
-
 ?
3.2.1.35 chondroitin 6-sulfate + H2O
-
 Bos taurus ?
-
 ?
3.2.1.35 hyaluronan + H2O HPLC and MS analyses of the products reveal the existance of oligosaccharides which are not produced under hydrolysis conditions, but during transglycosylation by the enzyme, suggesting that a novel enzyme is present as a contaminant in commercial BTH, which acts as an N-deacetylase of N-acetylglucosamine at the reducing terminal of hyaluronan oligosaccharides Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 BTH
-
 Bos taurus
3.2.1.35 hyaluronidase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 6
-
 assay at Bos taurus