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Literature summary extracted from

  • Ashida, H.; Maki, R.; Ozawa, H.; Tani, Y.; Kiyohara, M.; Fujita, M.; Imamura, A.; Ishida, H.; Kiso, M.; Yamamoto, K.
    Characterization of two different endo-alpha-N-acetylgalactosaminidases from probiotic and pathogenic enterobacteria, Bifidobacterium longum and Clostridium perfringens (2008), Glycobiology, 18, 727-734.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.97 EngCP expressed in Escherichia coli Clostridium perfringens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.97 Cu2+ is severely inhibited by 1 mM Clostridium perfringens
3.2.1.97 additional information EngBF is not significantly inhibited by divalent cations Bifidobacterium longum
3.2.1.97 additional information 1 mM of Ca2+, Zn2+, Co2+, Mn2+, Mg2+, and Fe2+, or the chelating reagent EDTA have no effect on activity Clostridium perfringens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.97 0.051
-
 Galbeta1-3GalNAcalpha1-pNP
-
 Clostridium perfringens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.97 Bifidobacterium longum
-
-
-
3.2.1.97 Clostridium perfringens Q8XMJ5
-
-
3.2.1.97 Clostridium perfringens 13 Q8XMJ5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.97 4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O 1.8% relative activity Clostridium perfringens 4-nitrophenol + N-acetyl-alpha-D-galactosamine
-
 ?
3.2.1.97 4-nitrophenyl 2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside + H2O 1.8% relative activity Clostridium perfringens 13 4-nitrophenol + N-acetyl-alpha-D-galactosamine
-
 ?
3.2.1.97 asialofetuin + H2O
-
 Clostridium perfringens ?
-
 ?
3.2.1.97 asialofetuin + H2O
-
 Clostridium perfringens 13 ?
-
 ?
3.2.1.97 Galbeta1-3(GlcNAcbeta1-6)GalNAcalpha1-pNP + H2O 2.3% relative activity Clostridium perfringens ?
-
 ?
3.2.1.97 Galbeta1-3(GlcNAcbeta1-6)GalNAcalpha1-pNP + H2O 2.3% relative activity Clostridium perfringens 13 ?
-
 ?
3.2.1.97 Galbeta1-3GalNAcalpha1-pNP + H2O 100% relative activity Bifidobacterium longum ?
-
 ?
3.2.1.97 Galbeta1-3GalNAcalpha1-pNP + H2O 100% relative activity Clostridium perfringens ?
-
 ?
3.2.1.97 Galbeta1-3GalNAcalpha1-pNP + H2O 100% relative activity Clostridium perfringens 13 ?
-
 ?
3.2.1.97 GalNAcbeta1-3GalNAcalpha1-pNP + H2O 17.3% relative activity Bifidobacterium longum ?
-
 ?
3.2.1.97 GalNAcbeta1-3GalNAcalpha1-pNP + H2O 21% relative activity Clostridium perfringens ?
-
 ?
3.2.1.97 gastric mucin + H2O EngBF does not release oligosaccharides from intact gastric mucin, but releases core 1 disaccharide from the gastric mucin pretreated with commercial sialidase and with the recombinant exo-alpha-N-acetylglucosamindase Bifidobacterium longum disaccharide + ?
-
 ?
3.2.1.97 gastric mucin + H2O EngCP does not release oligosaccharides from intact gastric mucin, but releases core 1 disaccharide from the gastric mucin pretreated with commercial sialidase and with the recombinant exo-alpha-N-acetylglucosamindase cloned from the same strain of Clostridium perfringens. It does not release core 2 trisaccharide from the mucin Clostridium perfringens disaccharide + ?
-
 ?
3.2.1.97 Glcbeta1-3GalNAcalpha1-pNP + H2O 156.7% relative activity Clostridium perfringens ?
-
 ?
3.2.1.97 Glcbeta1-3GalNAcalpha1-pNP + H2O 35.3% relative activity Bifidobacterium longum ?
-
 ?
3.2.1.97 GlcNAcbeta1-3GalNAcalpha1-pNP + H2O 0.3% relative activity Bifidobacterium longum ?
-
 ?
3.2.1.97 GlcNAcbeta1-3GalNAcalpha1-pNP + H2O 3.5% relative activity Clostridium perfringens ?
-
 ?
3.2.1.97 additional information EngCP from Clostridium perfringens possesses broader substrate specificity than EngBF Bifidobacterium longum ?
-
 ?
3.2.1.97 additional information EngCP possesses broader substrate specificity than EngBF of Bifidobacterium longum, which may contribute to the pathogenicity of Clostridium perfringens. EngCP does not act on sialyl core 1 O-glycans, e.g., fetuin Clostridium perfringens ?
-
 ?
3.2.1.97 additional information EngCP possesses broader substrate specificity than EngBF of Bifidobacterium longum, which may contribute to the pathogenicity of Clostridium perfringens. EngCP does not act on sialyl core 1 O-glycans, e.g., fetuin Clostridium perfringens 13 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.97 endo-alpha-GalNAc-ase
-
 Bifidobacterium longum
3.2.1.97 endo-alpha-GalNAc-ase
-
 Clostridium perfringens
3.2.1.97 endo-alpha-N-acetylgalactosaminidase
-
 Bifidobacterium longum
3.2.1.97 endo-alpha-N-acetylgalactosaminidase
-
 Clostridium perfringens
3.2.1.97 EngBF
-
 Bifidobacterium longum
3.2.1.97 EngCP
-
 Clostridium perfringens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.97 60
-
-
 Clostridium perfringens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.97 110
-
 Galbeta1-3GalNAcalpha1-pNP
-
 Clostridium perfringens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.97 additional information
-
 optimal pH between pH 5-6 for all hydrolyzable substrates of EngBF Bifidobacterium longum
3.2.1.97 6
-
 optimum pH of the enzyme for Galbeta1-3GalNAcalpha1-pNP hydrolysis is at pH 6.0 and 9.0, whereas optimum pH for other substrates hydrolyzed is around pH 6.0 Clostridium perfringens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.97 6 9 retains more than 80% activity Clostridium perfringens