BRENDA - Enzyme Database

Glutathionylation regulates cytosolic NADP+-dependent isocitrate dehydrogenase activity

Shin, S.W.; Oh, C.J.; Kil, I.S.; Park, J.W.; Free Radic. Res. 43, 409-416 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
expressed in Escherichia coli
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
C269S
activity of the C269S mutant is not affected by glutathione disulfide and no glutathionylated IDPc is observed with 5 mM glutathione disulfide confirming that Cys269 is a target of IDPc glutathionylation
Homo sapiens
1.1.1.42
C379S
glutathionylation of the C379S mutant is similar to that of wild type enzyme
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
Diamide
-
Homo sapiens
1.1.1.42
glutathione disulfide
incubation with 5 mM glutathione disulfide for 30 min completely eliminates activity
Homo sapiens
1.1.1.42
additional information
5 mM glutathione does not noticeably inhibit IDPc activity
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
45000
-
SDS-PAGE
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Homo sapiens
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.1.1.42
glutathionylation
Cys269 of IDPc is a target for S-glutathionylation, this modification is reversed by dithiothreitol as well as enzymatically by cytosolic glutaredoxin in the presence of glutathione. Glutathionylated IDPc is significantly less susceptible than native protein to peptide fragmentation by reactive oxygen species and proteolytic digestion. Glutathionylation may play a protective role in the degradation of protein through the structural alterations of IDPc.
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
Ni-NTA column chromatography
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
HEK-293 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
698059
Homo sapiens
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
expressed in Escherichia coli
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
C269S
activity of the C269S mutant is not affected by glutathione disulfide and no glutathionylated IDPc is observed with 5 mM glutathione disulfide confirming that Cys269 is a target of IDPc glutathionylation
Homo sapiens
1.1.1.42
C379S
glutathionylation of the C379S mutant is similar to that of wild type enzyme
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
Diamide
-
Homo sapiens
1.1.1.42
glutathione disulfide
incubation with 5 mM glutathione disulfide for 30 min completely eliminates activity
Homo sapiens
1.1.1.42
additional information
5 mM glutathione does not noticeably inhibit IDPc activity
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
45000
-
SDS-PAGE
Homo sapiens
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.1.1.42
glutathionylation
Cys269 of IDPc is a target for S-glutathionylation, this modification is reversed by dithiothreitol as well as enzymatically by cytosolic glutaredoxin in the presence of glutathione. Glutathionylated IDPc is significantly less susceptible than native protein to peptide fragmentation by reactive oxygen species and proteolytic digestion. Glutathionylation may play a protective role in the degradation of protein through the structural alterations of IDPc.
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
Ni-NTA column chromatography
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
HEK-293 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
698059
Homo sapiens
2-oxoglutarate + CO2 + NADPH
-
-
-
?