EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.9 | expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 0.0032 | - |
thioredoxin disulfide | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
1.8.1.9 | 0.0036 | - |
thioredoxin disulfide | purified, recombinant, dimeric enzyme | Rattus norvegicus | |
1.8.1.9 | 0.139 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
1.8.1.9 | 0.2156 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, dimeric enzyme | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | selenium | TrxR1 is a selenoprotein that contains a selenocysteine residue at the penultimate C-terminal | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.9 | 54386 | - |
2 * 54386, highly active enzyme form, calculated from amino acid sequence | Rattus norvegicus |
1.8.1.9 | 150000 | - |
highly active enzyme form, gel filtration | Rattus norvegicus |
1.8.1.9 | 250000 | - |
low-activity enzyme form, gel filtration | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.9 | Rattus norvegicus | O89049 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.9 | 2',5'-ADP Sepharose column chromatography and phenylarsine oxide Sepharose column chromatography | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.9 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Rattus norvegicus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.9 | thioredoxin disulfide + NADPH + H+ | - |
Rattus norvegicus | thioredoxin + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.9 | homodimer | 2 * 54386, highly active enzyme form, calculated from amino acid sequence | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.9 | thioredoxin reductase 1 | - |
Rattus norvegicus |
1.8.1.9 | TrxR1 | - |
Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 33.08 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
1.8.1.9 | 47 | 73 | thioredoxin disulfide | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
1.8.1.9 | 106.3 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, dimeric enzyme | Rattus norvegicus | |
1.8.1.9 | 114.1 | - |
thioredoxin disulfide | purified, recombinant, dimeric enzyme | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.9 | FAD | - |
Rattus norvegicus | |
1.8.1.9 | NADPH | - |
Rattus norvegicus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.9 | 183.3 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
1.8.1.9 | 495 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | purified, recombinant, dimeric enzyme | Rattus norvegicus | |
1.8.1.9 | 14920 | - |
thioredoxin disulfide | purified, recombinant, tetrameric enzyme | Rattus norvegicus | |
1.8.1.9 | 31700 | - |
thioredoxin disulfide | purified, recombinant, dimeric enzyme | Rattus norvegicus |