EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.11 | H2O2 | - |
Leishmania major |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.11.1.11 | into the vector pXG-B2863 for transfection of Leishmania major cells | Leishmania major |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.11 | azide | - |
Leishmania major |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.11.1.11 | mitochondrial intermembrane space | - |
Leishmania major | 5758 | - |
1.11.1.11 | mitochondrion | - |
Leishmania major | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.11.1.11 | 30000 | - |
determined by SDS-PAGE and Western blot analysis, LmAPX in the intermembrane space of the mitochondrial inner membrane is appr. 3.6 kDa shorter than the full-length gene | Leishmania major |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.11 | L-ascorbate + H2O2 | Leishmania major | - |
dehydroascorbate + H2O | - |
? | |
1.11.1.11 | L-ascorbate + H2O2 | Leishmania major 5ASKH | - |
dehydroascorbate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.11.1.11 | Leishmania major | - |
- |
- |
1.11.1.11 | Leishmania major 5ASKH | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.11.1.11 | mitochondria are prepared and fractionated | Leishmania major |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.11.1.11 | dihydrorhodamine 123 + H2O2 | assay, peroxidase substrate | Leishmania major | ? | - |
? | |
1.11.1.11 | dihydrorhodamine 123 + H2O2 | assay, peroxidase substrate | Leishmania major 5ASKH | ? | - |
? | |
1.11.1.11 | L-ascorbate + H2O2 | - |
Leishmania major | dehydroascorbate + H2O | - |
? | |
1.11.1.11 | L-ascorbate + H2O2 | - |
Leishmania major 5ASKH | dehydroascorbate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.11.1.11 | ascorbate peroxidase | - |
Leishmania major |
1.11.1.11 | LmAPX | - |
Leishmania major |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.11.1.11 | 30 | - |
peroxidase assay | Leishmania major |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.11.1.11 | heme | - |
Leishmania major |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.11.1.11 | Leishmania major | expression of LmAPX is increased when cells are treated with exogenous H2O2 | up |