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Literature summary extracted from

  • Liau, Y.; Chen, Y.; Lin, C.; Huang, J.; Lin, C.
    Characterisation of 2-Cys peroxiredoxin isozyme (Prx1) from Taiwanofungus camphorata (Niu-chang-chih): Expression and enzyme properties (2010), Food Chem., 119, 154-160.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.24 dithiothreitol dithiothreitol is capable of reducing the oxidised form of the Prx1 to regenerate its activity Taiwanofungus camphoratus
1.11.1.24 additional information Prx1's ability to remove H2O2 is not supported by the presence of 1 mM GSH Taiwanofungus camphoratus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expressed in Escherichia coli C43(DE3) cells Taiwanofungus camphoratus

General Stability

EC Number General Stability Organism
1.11.1.24 the enzyme shows 57% activity after 40 min of incubation at 37°C with trypsin, the enzyme shows 46% activity after 20 min of incubation at 37°C with one-tenth its weight of chymotrypsin, the enzyme retains about 45% activity in 0.2 M imidazole Taiwanofungus camphoratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 23000
-
 Prx1 monomer, SDS-PAGE Taiwanofungus camphoratus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.11.1.24 SDS the enzyme retains 40.5% activity in 2% (w/v) SDS Taiwanofungus camphoratus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Taiwanofungus camphoratus A1KXR1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 Ni-NTA Sepharose column chromatography Taiwanofungus camphoratus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 fruit body
-
 Taiwanofungus camphoratus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + reduced thioredoxin
-
 Taiwanofungus camphoratus H2O + oxidized thioredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 Prx1 2-Cys peroxiredoxin isozyme Taiwanofungus camphoratus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.24 60
-
 the enzyme retains 60% activity at 60°C for 2.5 min Taiwanofungus camphoratus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.24 5 11 the enzyme is stable under a broad pH range from 5.0 to 11.0 Taiwanofungus camphoratus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.24 thioredoxin
-
 Taiwanofungus camphoratus