EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | 1,4-dithiothreitol | complete inhibition at 10 mM | Beta vulgaris | |
1.14.18.1 | ascorbic acid | completely inhibited by 10 mM ascorbic acid | Beta vulgaris | |
1.14.18.1 | beta-mercaptoethanol | complete inhibition at 10 mM | Beta vulgaris | |
1.14.18.1 | Ca2+ | 65% residual activity at 10 mM | Beta vulgaris | |
1.14.18.1 | cetyl trimethylammonium bromide | 46% residual activity at 0.2% (v/v) | Beta vulgaris | |
1.14.18.1 | Citric acid | 65% residual activity at 10 mM | Beta vulgaris | |
1.14.18.1 | Co2+ | 75% residual activity at 10 mM | Beta vulgaris | |
1.14.18.1 | Cu2+ | 50% residual activity at 1 mM | Beta vulgaris | |
1.14.18.1 | cysteine | complete inhibition at 10 mM | Beta vulgaris | |
1.14.18.1 | EDTA | 81% residual activity at 10 mM | Beta vulgaris | |
1.14.18.1 | Mg2+ | 65% residual activity at 10 mM | Beta vulgaris | |
1.14.18.1 | Mn2+ | 75% residual activity at 10 mM | Beta vulgaris | |
1.14.18.1 | Sodium azide | 83% residual activity at 10 mM | Beta vulgaris | |
1.14.18.1 | Sodium diethyldithiocarbamate | complete inhibition at 10 mM | Beta vulgaris | |
1.14.18.1 | Sodium metabisulfite | complete inhibition at 10 mM | Beta vulgaris | |
1.14.18.1 | sodium sulfite | complete inhibition at 10 mM | Beta vulgaris | |
1.14.18.1 | Thiourea | complete inhibition at 10 mM | Beta vulgaris | |
1.14.18.1 | Tween 20 | 84% residual activity at 10 mM | Beta vulgaris | |
1.14.18.1 | Zn2+ | 77% residual activity at 10 mM | Beta vulgaris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.18.1 | K+ | 127% activity at 1 mM | Beta vulgaris | |
1.14.18.1 | Na+ | 114% activity at 10 mM | Beta vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 41000 | - |
SDS-PAGE | Beta vulgaris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.18.1 | Beta vulgaris | - |
red Swiss chard, subspecies Beta vulgaris cicla | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.18.1 | ammonium sulfate fractionation, DEAE-Sepharose column chromatography and Sephadex G-75 gel filtration | Beta vulgaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.18.1 | leaf | - |
Beta vulgaris | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.18.1 | 2 catechol + O2 | 86% relative activity compared to L-DOPA | Beta vulgaris | 2 1,2-benzoquinone + 2 H2O | - |
? | |
1.14.18.1 | 2,3-dimethylphenol + O2 | 11% relative activity compared to L-DOPA | Beta vulgaris | ? | - |
? | |
1.14.18.1 | 4-methoxyphenol + O2 | 40% relative activity compared to L-DOPA, weak monophenolase activity | Beta vulgaris | ? | - |
? | |
1.14.18.1 | chlorogenic acid + O2 | 80% relative activity compared to L-DOPA | Beta vulgaris | ? | - |
? | |
1.14.18.1 | gallic acid + O2 | 73% relative activity compared to L-DOPA | Beta vulgaris | ? | - |
? | |
1.14.18.1 | L-Dopa + O2 | 100% activity | Beta vulgaris | L-dopaquinone + H2O | - |
? | |
1.14.18.1 | L-tyrosine + O2 | 2% relative activity compared to L-DOPA, weak monophenolase activity | Beta vulgaris | L-DOPA + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.18.1 | monomer | 1 * 41000, SDS-PAGE | Beta vulgaris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.18.1 | polyphenol oxidase | - |
Beta vulgaris |
1.14.18.1 | PPO | - |
Beta vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 45 | - |
- |
Beta vulgaris |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 30 | 70 | about 55% of maximal activity at 30°C and at 70°C | Beta vulgaris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 10 | 70 | the activity of recombinant PPO gradually increases with temperature ranging from 10 to 45°C, but followed by a decrease at higher temperatures, the residual soluble PPO activity remains above 60% after 40 min at 30, 40 and 50°C and then decreases significantly when temperature reaches at 60°C, with only 55% activity in just 10 min. When the temperature is raised to 80°C, the enzyme retains less than 18% of its original activity after 20 min and even less after prolonged incubation. | Beta vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 7.5 | - |
- |
Beta vulgaris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 6.5 | 10 | pH 6.5: about 60% of maximal activity, pH 10.0: about 10% of maximal activity | Beta vulgaris |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.18.1 | 3 | 10 | stable between pH 4.0 and 10.0 retaining more than 80% of its activity in the pH range of 5.0-9.0, PPO is rapidly inactivated by the pH below 5.0, the activity of recombinant PPO gradually increases with pH value in the range of 5-7, the activity decreases rapidly below and above pH 7.5, more than 70% of the optimal activity is kept between pH 7.5 and 10.0, after incubating at pH 11.0 for 1 h the recombinant PPO still retains 74% of its activity whereas at pH 3.0 it is left with only 39.4% | Beta vulgaris |