EC Number | Application | Comment | Organism |
---|---|---|---|
1.8.1.2 | brewing | mutations in the sulfite reductase genes, MET5 and MET10, are responsible for low H2S phenotypes, yeast strains with reduced H2S production offer promising solutions to H2S-related taints generated during wine production | Saccharomyces cerevisiae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.2 | the MET5 and Met10 coding sequences are cloned into the vector pGEM-T-easy and subsequently into the centromeric Saccharomyces cerevisiae expression plasmid pRS416 | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.2 | A979T | Met5p mutant | Saccharomyces cerevisiae |
1.8.1.2 | E1356K | Met5p mutant | Saccharomyces cerevisiae |
1.8.1.2 | E929K | Met10p mutant | Saccharomyces cerevisiae |
1.8.1.2 | G1115D | Met5p mutant | Saccharomyces cerevisiae |
1.8.1.2 | L606F | Met10p mutant | Saccharomyces cerevisiae |
1.8.1.2 | T990I | Met10p mutant | Saccharomyces cerevisiae |
1.8.1.2 | T997I | Met10p mutant | Saccharomyces cerevisiae |
1.8.1.2 | W59X | Met10p mutant | Saccharomyces cerevisiae |
1.8.1.2 | W841X | Met10p mutant | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.2 | hydrogen sulfide + NADP+ + H2O | Saccharomyces cerevisiae | - |
sulfite + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.2 | Saccharomyces cerevisiae | - |
allele 1, subunit beta, MET5, subunit alpha, MET10 | - |
1.8.1.2 | Saccharomyces cerevisiae | - |
allele 2, subunit beta, MET5, subunit alpha, MET10 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.2 | hydrogen sulfide + NADP+ + H2O | - |
Saccharomyces cerevisiae | sulfite + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.2 | heterotetramer | two alpha- and two beta-subunits, encoded by the MET10 and MET5 gene, respectively | Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.2 | sulfite reductase | - |
Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.2 | FAD | - |
Saccharomyces cerevisiae | |
1.8.1.2 | FMN | - |
Saccharomyces cerevisiae | |
1.8.1.2 | iron-sulfur centre | - |
Saccharomyces cerevisiae | |
1.8.1.2 | NADPH | - |
Saccharomyces cerevisiae | |
1.8.1.2 | siroheme | - |
Saccharomyces cerevisiae |