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Literature summary extracted from

  • Quentmeier, A.; Antranikian, G.
    Characterization of citrate lyase from Clostridium sporosphaeroides (1985), Arch. Microbiol., 141, 85-90.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.3.6 L-Glu addition of L-Glu is not necessary for activity and stabilization of the enzyme [Clostridium] sporosphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.6 0.24
-
citrate
-
[Clostridium] sporosphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.6 Co2+ divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+ [Clostridium] sporosphaeroides
4.1.3.6 Cu2+ divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+ [Clostridium] sporosphaeroides
4.1.3.6 Mg2+ divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+, Mg2+ is most effective [Clostridium] sporosphaeroides
4.1.3.6 Mn2+ divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+ [Clostridium] sporosphaeroides
4.1.3.6 Zn2+ divalent cation required: Mg2+, Zn2+, Mn2+, Co2+ or Cu2+ [Clostridium] sporosphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.6 11800
-
x * 11800, + x * 34000 + x * 56600, SDS-PAGE [Clostridium] sporosphaeroides
4.1.3.6 34000
-
x * 11800, + x * 34000 + x * 56600, SDS-PAGE [Clostridium] sporosphaeroides
4.1.3.6 56600
-
x * 11800, + x * 34000 + x * 56600, SDS-PAGE [Clostridium] sporosphaeroides
4.1.3.6 536600
-
gel filtration, sedimentation equilibrium centrifugation [Clostridium] sporosphaeroides

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.6 [Clostridium] sporosphaeroides
-
-
-
6.2.1.22 [Clostridium] sporosphaeroides
-
DSM 1294
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.6
-
[Clostridium] sporosphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.6 additional information
-
-
[Clostridium] sporosphaeroides
4.1.3.6 34.1
-
-
[Clostridium] sporosphaeroides

Storage Stability

EC Number Storage Stability Organism
4.1.3.6 4°C, pH 7.2, 100 mM potassium phosphate buffer, 3 mM MgCl2, 1 mM dithiothreitol, stable for 3 days [Clostridium] sporosphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.6 citrate
-
[Clostridium] sporosphaeroides acetate + oxaloacetate
-
?
6.2.1.22 ATP + acetate + citrate (pro-3S)-lyase thiol form of (pro-3S)-lyase [Clostridium] sporosphaeroides AMP + diphosphate + citrate(pro-3S)-lyase
-
?

Subunits

EC Number Subunits Comment Organism
4.1.3.6 oligomer x * 11800, + x * 34000 + x * 56600, SDS-PAGE [Clostridium] sporosphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.6 50
-
-
[Clostridium] sporosphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.6 30
-
24 h, 7% loss of activity [Clostridium] sporosphaeroides
4.1.3.6 60
-
15 min, 9% loss of activity [Clostridium] sporosphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.6 6.8 8 in 100 mM tris-HCl buffer or 100 mM potassium phosphate buffer [Clostridium] sporosphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.6 6.8 9 pH 6.8-8.0: pH-optimum, pH 9.0: more than 80% of maximal activity [Clostridium] sporosphaeroides