Literature summary extracted from
Saito, A.; Ooya, T.; Miyatsuchi, D.; Fuchigami, H.; Terakado, K.; Nakayama, S.Y.; Watanabe, T.; Nagata, Y.; Ando, A.
Molecular characterization and antifungal activity of a family 46 chitosanase from Amycolatopsis sp. CsO-2 (2009), FEMS Microbiol. Lett., 293, 79-84.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.132 |
wild-type and mutant overexpressed from vector pET15b/CSO2 in Escherichia coli BL21(DE3) |
Amycolatopsis sp. CsO-2 |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.2.1.132 |
E22Q |
abolished chitosanase activity, does not exhibit antifungal activity |
Amycolatopsis sp. CsO-2 |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.2.1.132 |
additional information |
antifungal effect of CtoA against Rhizopus oryzae IAM6252 is drastically enhanced by the simultaneous addition of the family 19 chitinase ChiC from Streptomyces griseus |
Amycolatopsis sp. CsO-2 |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.2.1.132 |
26490 |
- |
sequence analysis |
Amycolatopsis sp. CsO-2 |
3.2.1.132 |
30000 |
- |
1 * 30000, SDS-PAGE |
Amycolatopsis sp. CsO-2 |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.132 |
Amycolatopsis sp. CsO-2 |
Q9LBG4 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.132 |
to homogeneity |
Amycolatopsis sp. CsO-2 |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.2.1.132 |
chitosan + H2O |
exhibits antifungal activity against Rhizopus oryzae IAM6252, which is known to produce chitosan probably as a cell wall component. Glu22 acts as an active center in the CtoA protein |
Amycolatopsis sp. CsO-2 |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.2.1.132 |
monomer |
1 * 30000, SDS-PAGE |
Amycolatopsis sp. CsO-2 |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.132 |
CtoA |
- |
Amycolatopsis sp. CsO-2 |
3.2.1.132 |
family 46 chitosanase |
- |
Amycolatopsis sp. CsO-2 |