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Literature summary extracted from

  • Saito, A.; Ooya, T.; Miyatsuchi, D.; Fuchigami, H.; Terakado, K.; Nakayama, S.Y.; Watanabe, T.; Nagata, Y.; Ando, A.
    Molecular characterization and antifungal activity of a family 46 chitosanase from Amycolatopsis sp. CsO-2 (2009), FEMS Microbiol. Lett., 293, 79-84.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 wild-type and mutant overexpressed from vector pET15b/CSO2 in Escherichia coli BL21(DE3) Amycolatopsis sp. CsO-2

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 E22Q abolished chitosanase activity, does not exhibit antifungal activity Amycolatopsis sp. CsO-2

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 additional information antifungal effect of CtoA against Rhizopus oryzae IAM6252 is drastically enhanced by the simultaneous addition of the family 19 chitinase ChiC from Streptomyces griseus Amycolatopsis sp. CsO-2

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 26490
-
sequence analysis Amycolatopsis sp. CsO-2
3.2.1.132 30000
-
1 * 30000, SDS-PAGE Amycolatopsis sp. CsO-2

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Amycolatopsis sp. CsO-2 Q9LBG4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 to homogeneity Amycolatopsis sp. CsO-2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O exhibits antifungal activity against Rhizopus oryzae IAM6252, which is known to produce chitosan probably as a cell wall component. Glu22 acts as an active center in the CtoA protein Amycolatopsis sp. CsO-2 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 monomer 1 * 30000, SDS-PAGE Amycolatopsis sp. CsO-2

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 CtoA
-
Amycolatopsis sp. CsO-2
3.2.1.132 family 46 chitosanase
-
Amycolatopsis sp. CsO-2