Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chouayekh, H.; Serror, P.; Boudebbouze, S.; Maguin, E.
    Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene (2009), FEMS Microbiol. Lett., 293, 232-239.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.31.1 analysis staphylococcal nuclease fused at its N-terminus to signal peptide of the lactococcal Usp45 protein (SP Usp45-NucB), as reporter for expression and secretion in Lactobacillus bulgaricus Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.31.1 staphylococcal nuclease fused at its N-terminus to signal peptide of the lactococcal Usp45 protein (SP Usp45-NucB), as reporter for expression and secretion in Lactobacillus bulgaricus, SDS PAGE and western blot of culture supernatant and cell lysate used for analysis Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.31.1 additional information
-
 additional information 1250 microg DNA/ml, construct phlbA-sp usp45-nuc, promotor phlbA, lactococcal signal peptide Usp45: sp usp45 Staphylococcus aureus
3.1.31.1 additional information
-
 additional information 430 microg DNA/ml, construct pusp45-sp usp45-nuc, promoter: pusp45, lactococcal signal peptide Usp45: sp usp45 Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.31.1 Ca2+
-
 Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.1.31.1 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.31.1 DNA + H2O
-
 Staphylococcus aureus 3'-deoxymononucleotides + dinucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.31.1 staphylococcus aureus nuclease Nuc, named NucB when secreted as an active 168-amino acid-long polypeptide Staphylococcus aureus