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Literature summary extracted from

  • Mehboob, F.; Wolterink, A.F.; Vermeulen, A.J.; Jiang, B.; Hagedoorn, P.L.; Stams, A.J.; Kengen, S.W.
    Purification and characterization of a chlorite dismutase from Pseudomonas chloritidismutans (2009), FEMS Microbiol. Lett., 293, 115-121.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.49 azide at 70 microM 44% inhibition, at 700 microM 89% inhibition Stutzerimonas chloritidismutans
1.13.11.49 cyanide at 70 microM 83% inhibition, at 700 microM 95% inhibition Stutzerimonas chloritidismutans
1.13.11.49 EDTA at 70 microM 13% inhibition, at 700 microM 30% inhibition Stutzerimonas chloritidismutans
1.13.11.49 H2O2 at 70 microM 6% inhibition, at 700 microM 37% inhibition Stutzerimonas chloritidismutans
1.13.11.49 additional information excess chlorite inactivates and bleaches the enzyme, 80% activity loss with 56 mM chlorite, 90% after second application of 56 mM chlorite; no inhibition by hydroxylamine or 3-amino-1,2,3-triazole Stutzerimonas chloritidismutans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.49 periplasm
-
 Stutzerimonas chloritidismutans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.49 (NH4)2SO4 activity increases with increasing salt concentration up to 2 M Stutzerimonas chloritidismutans
1.13.11.49 additional information kosmotropic salts such as sulfate and phosphate stabilize protein structures by water-structuring effects of the ions, chaotropic salts such as nitrate and chlorate reduce the activity, no effect of NaCl, NH4Cl, and NaHCO3 (up to 1.5 M) on enzyme activity Stutzerimonas chloritidismutans
1.13.11.49 Na2SO4 activity increases with increasing salt concentration up to 2 M Stutzerimonas chloritidismutans
1.13.11.49 NaClO3 decreasing activity with increasing salt concentration Stutzerimonas chloritidismutans
1.13.11.49 NaNO3 decreasing activity with increasing salt concentration Stutzerimonas chloritidismutans
1.13.11.49 potassium phosphate activity increases with increasing salt concentration up to 1 M salt, decrease beyond 1 M salt Stutzerimonas chloritidismutans
1.13.11.49 Sodium phosphate activity increases with increasing salt concentration up to 1 M salt, decrease beyond 1 M salt Stutzerimonas chloritidismutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.49 31000
-
 4 * 31000, heme content 1.5 mol Fe2+/mol enzyme (calculated from Soret peak shift measurement), 2.2 mol Fe2+/mol enzyme (metal analysis by ICP-MS) Stutzerimonas chloritidismutans
1.13.11.49 110000
-
 SDS-PAGE, native enzyme with Superdex 200 column with 50 mM potassium phosphate buffer, pH 7.0 and 100 mM NaCl Stutzerimonas chloritidismutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.49 chlorite Stutzerimonas chloritidismutans
-
 chloride + O2 water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) ?
1.13.11.49 chlorite Stutzerimonas chloritidismutans AW-1 / DSM 13592
-
 chloride + O2 water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.49 Stutzerimonas chloritidismutans
-
-
-
1.13.11.49 Stutzerimonas chloritidismutans AW-1 / DSM 13592
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.49 cell pellet suspended in 0.05 M Tris-HCl buffer, pH 9.5 and 0.05 EDTA for 30 min at room temperature, centrifuged, supernatant ultracentrifuged, red supernatant containing periplasmic fraction separated from pellet, diluted with 10 mM Tris-HCl buffer, pH 7.2, purification with ÄKTAfplc, diluted supernatant loaded onto hydroxyapatite column with 10 mM Tris-HCl, pH 7.2, enzyme elutes at the start of a linear gradient of 10 mM Tris-Hcl, pH 7.2 to 450 mM potassium phosphate, pH 7.2, storage at -20°C Stutzerimonas chloritidismutans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.49 additional information
-
 the formation of O2 is influenced by the concentrations of enzyme and chlorite in 1 M phosphate buffer, pH 6.0, at 25°C, undiluted chlorite dismutase (0.64 mg/l) produces 98 microM O2 with 100 microM ClO2-, 205 microM O2 with 200 microM ClO2-, 300 microM O2 with 300 microM ClO2-, 10 times diluted chlorite dismutase (0.064 mg/l) produces 34 microM O2 with 100 microM ClO2-, 69 microM O2 with 200 microM ClO2-, 96 microM O2 with 300 microM ClO2- Stutzerimonas chloritidismutans
1.13.11.49 443
-
 Vmax, 100 mM sodium phosphate buffer, pH 6.0, 25°C, 0.2 mM chlorite Stutzerimonas chloritidismutans
1.13.11.49 4650
-
 Vmax increase with 1 M phosphate, pH 6.0, 25°C Stutzerimonas chloritidismutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.49 chlorite
-
 Stutzerimonas chloritidismutans chloride + O2 water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) ?
1.13.11.49 chlorite
-
 Stutzerimonas chloritidismutans AW-1 / DSM 13592 chloride + O2 water is not involved in the O2-building reaction (no inclusion of 18O-isotope from labeled water) ?

Subunits

EC Number Subunits Comment Organism
1.13.11.49 homotetramer 4 * 31000, heme content 1.5 mol Fe2+/mol enzyme (calculated from Soret peak shift measurement), 2.2 mol Fe2+/mol enzyme (metal analysis by ICP-MS) Stutzerimonas chloritidismutans

Synonyms

EC Number Synonyms Comment Organism
1.13.11.49 chlorite dismutase
-
 Stutzerimonas chloritidismutans
1.13.11.49 CLD
-
 Stutzerimonas chloritidismutans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.49 25
-
 100 mM sodium phosphate buffer, pH 7.2 Stutzerimonas chloritidismutans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.49 15 45 100 mM sodium phosphate buffer, pH 7.2 Stutzerimonas chloritidismutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.49 6
-
 sodium phosphate buffer, 25°C Stutzerimonas chloritidismutans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.49 5 8.5 sodium phosphate buffer, 25°C Stutzerimonas chloritidismutans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.49 2700
-
 Chlorite 100 mM sodium phosphate buffer, pH 6.0, 25°C Stutzerimonas chloritidismutans