Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • DiRienzo, J.M.; Cao, L.; Volgina, A.; Bandelac, G.; Korostoff, J..
    Functional and structural characterization of chimeras of a bacterial genotoxin and human type I DNAse (2009), FEMS Microbiol. Lett., 291, 222-231.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.1.21.1 medicine DNAse I/cdtB gene chimera has therapeutic applications for inhibiting the proliferation of cancer cells Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.1 DNAse I or DNAse I/cdtB gene chimera expressed from vectors pETBDN2 (cdB-DNAse I chimera), pETDNB1 (DNAse I-cdB chimera) and pETBDN2-1 to pETBDN2-5 (cdB-DNAse I mutated chimera) in Escherichia coli BL-21(DE3) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.1 G55R adds DNA contact residues, increases the specific activity of the hybrid protein Homo sapiens
3.1.21.1 L62E added a metal ion-binding residue, increases the specific activity of the hybrid protein Homo sapiens
3.1.21.1 R100E adds a residue that hydrogen bonds to the catalytic H160 in DNAse I, increases the specific activity of the hybrid protein Homo sapiens
3.1.21.1 S99Y adds DNA contact residues, increases the specific activity of the hybrid protein Homo sapiens
3.1.21.1 V134R adds DNA contact residues, increases the specific activity of the hybrid protein Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 actin inhibits the DNA-nicking activity of DNAse I/CdtB chimera Homo sapiens
3.1.21.1 additional information G-actin has no effect on the ability of CdtB/DNAse I chimera to convert supercoiled DNA to relaxed and linear forms Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Mg2+ required Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Bos taurus P00639
-
-
3.1.21.1 Homo sapiens P24855
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 pancreas
-
Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 DNA + H2O
-
Homo sapiens 5'-phosphooligonucleotides + ?
-
?
3.1.21.1 DNA + H2O DNAse I converts 100% of 1 microg of supercoiled plasmid DNA to relaxed form in 1 h at 37°C. A 10fold higher concentration of DNAse I converts 98% of 1 microg of supercoiled DNA to linear form and a 100fold higher concentration of the nuclease completely digests the DNA into small fragments Bos taurus 5'-phosphooligonucleotides + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.21.1 heterotrimer CdtB/DNAse I, but not DNAseI/CdtB Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.21.1 DNase I
-
Homo sapiens
3.1.21.1 DNase I
-
Bos taurus
3.1.21.1 type I DNAse I
-
Homo sapiens
3.1.21.1 type I DNAse I
-
Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.1 100
-
DNAse I DNA nicking-activity is less than 50% of the control after 5 min of heating Bos taurus