EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.21.1 | medicine | DNAse I/cdtB gene chimera has therapeutic applications for inhibiting the proliferation of cancer cells | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.21.1 | DNAse I or DNAse I/cdtB gene chimera expressed from vectors pETBDN2 (cdB-DNAse I chimera), pETDNB1 (DNAse I-cdB chimera) and pETBDN2-1 to pETBDN2-5 (cdB-DNAse I mutated chimera) in Escherichia coli BL-21(DE3) | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.21.1 | G55R | adds DNA contact residues, increases the specific activity of the hybrid protein | Homo sapiens |
3.1.21.1 | L62E | added a metal ion-binding residue, increases the specific activity of the hybrid protein | Homo sapiens |
3.1.21.1 | R100E | adds a residue that hydrogen bonds to the catalytic H160 in DNAse I, increases the specific activity of the hybrid protein | Homo sapiens |
3.1.21.1 | S99Y | adds DNA contact residues, increases the specific activity of the hybrid protein | Homo sapiens |
3.1.21.1 | V134R | adds DNA contact residues, increases the specific activity of the hybrid protein | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | actin | inhibits the DNA-nicking activity of DNAse I/CdtB chimera | Homo sapiens | |
3.1.21.1 | additional information | G-actin has no effect on the ability of CdtB/DNAse I chimera to convert supercoiled DNA to relaxed and linear forms | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.1 | Mg2+ | required | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.1 | Bos taurus | P00639 | - |
- |
3.1.21.1 | Homo sapiens | P24855 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.21.1 | pancreas | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.1 | DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
3.1.21.1 | DNA + H2O | DNAse I converts 100% of 1 microg of supercoiled plasmid DNA to relaxed form in 1 h at 37°C. A 10fold higher concentration of DNAse I converts 98% of 1 microg of supercoiled DNA to linear form and a 100fold higher concentration of the nuclease completely digests the DNA into small fragments | Bos taurus | 5'-phosphooligonucleotides + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.21.1 | heterotrimer | CdtB/DNAse I, but not DNAseI/CdtB | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.1 | DNase I | - |
Homo sapiens |
3.1.21.1 | DNase I | - |
Bos taurus |
3.1.21.1 | type I DNAse I | - |
Homo sapiens |
3.1.21.1 | type I DNAse I | - |
Bos taurus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.21.1 | 100 | - |
DNAse I DNA nicking-activity is less than 50% of the control after 5 min of heating | Bos taurus |