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Literature summary extracted from

  • Sello, J.K.; Buttner, M.J.
    The oligoribonuclease gene in Streptomyces coelicolor is not transcriptionally or translationally coupled to adpA, a key bldA target (2008), FEMS Microbiol. Lett., 286, 60-65.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.13.3 wild-type ornA and the ornA (D130A) point mutant are placed under the control of the strong, constitutive ermE promoter in the hygromycin-resistant, attP-integrative vector pIJ10275. The resultant plasmids (pJS93 and pJS94) introduced into Streptomyces coelicolor J3411 (DELTAornA::apr) by conjugation with Escherichia coli strain ET12567 (pUZ8002) to yield Streptomyces coelicolor J3411 (pJS93) and Streptomyces coelicolor J3411 (pJS94) Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
3.1.13.3 D130A pJS94, encoding the catalytically inactive point mutant OrnA D130A does not suppress the phenotype of the Streptomyces coelicolor J3411 (DELTAornA::apr) null mutant Streptomyces coelicolor
3.1.13.3 additional information oligoribonuclease null mutant J3411 (DELTAornA::apr), reveals a conditional defect in morphological differentiation that is reminiscent of bldA. PpJS93, containing ornA under the control of a constitutive promoter, completely suppresses the phenotype of Streptomyces coelicolor J3411 (DELTAornA::apr), but pJS94 (encoding catalytically inactive OrnA D130A) does not Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.13.3 additional information Streptomyces coelicolor degradation of RNA oligomers by oligoribonuclease is critical for completion of the Streptomyces coelicolor life cycle, but expression of ornA is not likely to be contigent on bldA-dependent translation of adpA ?
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Organism

EC Number Organism UniProt Comment Textmining
3.1.13.3 Streptomyces coelicolor
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.3 additional information degradation of RNA oligomers by oligoribonuclease is critical for completion of the Streptomyces coelicolor life cycle, but expression of ornA is not likely to be contigent on bldA-dependent translation of adpA Streptomyces coelicolor ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.13.3 oligoribonuclease
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 Streptomyces coelicolor