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Literature summary extracted from

  • Buchmeier, N.; Fahey, R.C.
    The mshA gene encoding the glycosyltransferase of mycothiol biosynthesis is essential in Mycobacterium tuberculosis Erdman (2006), FEMS Microbiol. Lett., 264, 74-79.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.250 construction of Mycobacterium tuberculosis with two copies of mshA Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate Mycobacterium tuberculosis 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, the mshA gene is essential for the growth of Mycobacterium tuberculosis 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate Mycobacterium tuberculosis Erdman 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, the mshA gene is essential for the growth of Mycobacterium tuberculosis 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.250 Mycobacterium tuberculosis
-
 Erdman
-
2.4.1.250 Mycobacterium tuberculosis Erdman
-
 Erdman
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.250 UDP-N-acetyl-alpha-D-glucosamine + 1D-myo-inositol 3-phosphate = 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, the mshA gene is essential for the growth of Mycobacterium tuberculosis Mycobacterium tuberculosis 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate Mycobacterium tuberculosis 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, the mshA gene is essential for the growth of Mycobacterium tuberculosis Mycobacterium tuberculosis Erdman 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?
2.4.1.250 UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate Mycobacterium tuberculosis Erdman 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP
-
 ?

General Information

EC Number General Information Comment Organism
2.4.1.250 malfunction inability to disrupt the mshA gene in Mycobacterium tuberculosis containing a single copy of mshA. Directed knock-out of the mshA gene in Mycobacterium tuberculosis Erdman is only possible when a second copy of mshA is first incorporated into the chromosome. Bacteria with only a single copy of mshA that grew after mutagenesis produced normal levels of mycothiol Mycobacterium tuberculosis