Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guranowski, A.; Wojdyla, A.M.; Pietrowska-Borek, M.; Bieganowski, P.; Khurs, E.N.; Cliff, M.J.; Blackburn, G.M.; Blaziak, D.; Stec, W.J.
    Fhit proteins can also recognize substrates other than dinucleoside polyphosphates (2008), FEBS Lett., 582, 3152-3158.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.29 gene At5g58240, DNA and amino acid sequence determination and analysis Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.29 adenosine 5'-O-(gamma-fluoromonophosphate)
-
 Arabidopsis thaliana
3.6.1.29 adenosine 5'-O-(gamma-fluorotriphosphate)
-
 Arabidopsis thaliana
3.6.1.29 adenosine 5'-phosphosulfate substrate inhibition Arabidopsis thaliana
3.6.1.29 adenosine 5'-phosphosulfate substrate inhibition Homo sapiens
3.6.1.29 ADP
-
 Arabidopsis thaliana
3.6.1.29 AMP product inhibition Arabidopsis thaliana
3.6.1.29 AMP product inhibition Homo sapiens
3.6.1.29 ATP
-
 Arabidopsis thaliana
3.6.1.29 additional information poor inhibition by fluoroadenylates, ATP, and ADP Homo sapiens
3.6.1.29 P1,P3-bis(5'-adenosyl) triphosphate substrate inhibition Arabidopsis thaliana
3.6.1.29 P1,P3-bis(5'-adenosyl) triphosphate substrate inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.29 0.003
-
 adenosine 5'-phosphoramidate pH 6.8, 30°C Homo sapiens
3.6.1.29 0.003
-
 5'-O-[amino(hydroxy)phosphoryl]adenosine pH 6.8, 30°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.29 Mg2+
-
 Arabidopsis thaliana
3.6.1.29 additional information no requirement for Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.29 adenosine 5'-phosphoramidate + H2O Homo sapiens
-
 AMP + NH3
-
 ?
3.6.1.29 adenosine 5'-phosphoramidate + H2O Arabidopsis thaliana no requirement for Mg2+ AMP + NH3
-
 ?
3.6.1.29 adenosine 5'-phosphosulfate + H2O Arabidopsis thaliana no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase AMP + sulfate
-
 ?
3.6.1.29 adenosine 5'-phosphosulfate + H2O Homo sapiens reaction of EC 3.6.2.1, adenylylsulfatase AMP + sulfate
-
 ?
3.6.1.29 adenylylfluoride + H2O Arabidopsis thaliana
-
 AMP + fluoride
-
 ?
3.6.1.29 gamma-fluoro-ATP + H2O Arabidopsis thaliana
-
 AMP + fluorodiphosphoric acid
-
 ?
3.6.1.29 additional information Arabidopsis thaliana Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview ?
-
 ?
3.6.1.29 additional information Homo sapiens Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview ?
-
 ?
3.6.1.29 P1,P3-bis(5'-adenosyl) triphosphate + H2O Homo sapiens
-
 ADP + AMP
-
 ?
3.6.1.29 P1,P3-bis(5'-adenosyl) triphosphate + H2O Arabidopsis thaliana requirement for Mg2+ ADP + AMP
-
 ?
3.6.1.29 P1-bis(5'-adenosyl) monophosphate + H2O Arabidopsis thaliana requirement for Mg2+ ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.29 Arabidopsis thaliana Q9LVM4 gene FHIT
-
3.6.1.29 Homo sapiens
-
 gene FHIT
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.29 additional information
-
 relative velocities of hydrolysis with different substrates, overview Homo sapiens
3.6.1.29 additional information
-
 relative velocities of hydrolysis with different substrates, overview Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.29 adenosine 5'-phosphoramidate + H2O
-
 Homo sapiens AMP + NH3
-
 ?
3.6.1.29 adenosine 5'-phosphoramidate + H2O preferred substrate Homo sapiens AMP + NH3
-
 ?
3.6.1.29 adenosine 5'-phosphoramidate + H2O preferred substrate Arabidopsis thaliana AMP + NH3
-
 ?
3.6.1.29 adenosine 5'-phosphoramidate + H2O no requirement for Mg2+ Arabidopsis thaliana AMP + NH3
-
 ?
3.6.1.29 adenosine 5'-phosphosulfate + H2O no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase Arabidopsis thaliana AMP + sulfate
-
 ?
3.6.1.29 adenosine 5'-phosphosulfate + H2O reaction of EC 3.6.2.1, adenylylsulfatase Homo sapiens AMP + sulfate
-
 ?
3.6.1.29 adenosine 5'-phosphosulfate + H2O reaction of EC 3.6.2.1, adenylylsulfatase Arabidopsis thaliana AMP + sulfate
-
 ?
3.6.1.29 adenylylfluoride + H2O
-
 Arabidopsis thaliana AMP + fluoride
-
 ?
3.6.1.29 gamma-fluoro-ATP + H2O
-
 Arabidopsis thaliana AMP + fluorodiphosphoric acid
-
 ?
3.6.1.29 inosine 5'-phosphoramidate + H2O
-
 Homo sapiens IMP + NH3
-
 ?
3.6.1.29 inosine 5'-phosphoramidate + H2O
-
 Arabidopsis thaliana IMP + NH3
-
 ?
3.6.1.29 additional information Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview Arabidopsis thaliana ?
-
 ?
3.6.1.29 additional information Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview Homo sapiens ?
-
 ?
3.6.1.29 additional information the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP Homo sapiens ?
-
 ?
3.6.1.29 additional information the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP Arabidopsis thaliana ?
-
 ?
3.6.1.29 P1,P3-bis(5'-adenosyl) triphosphate + H2O
-
 Homo sapiens ADP + AMP
-
 ?
3.6.1.29 P1,P3-bis(5'-adenosyl) triphosphate + H2O
-
 Arabidopsis thaliana ADP + AMP
-
 ?
3.6.1.29 P1,P3-bis(5'-adenosyl) triphosphate + H2O requirement for Mg2+ Arabidopsis thaliana ADP + AMP
-
 ?
3.6.1.29 P1-bis(5'-adenosyl) monophosphate + H2O
-
 Arabidopsis thaliana ?
-
 ?
3.6.1.29 P1-bis(5'-adenosyl) monophosphate + H2O requirement for Mg2+ Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.29 dinucleoside triphosphatase
-
 Homo sapiens
3.6.1.29 dinucleoside triphosphatase
-
 Arabidopsis thaliana
3.6.1.29 Fhit protein
-
 Homo sapiens
3.6.1.29 Fhit protein
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.29 30
-
 assay at Homo sapiens
3.6.1.29 30
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.29 1.26
-
 adenosine 5'-phosphoramidate pH 6.8, 30°C Homo sapiens
3.6.1.29 1.26
-
 5'-O-[amino(hydroxy)phosphoryl]adenosine pH 6.8, 30°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.29 6.8
-
 assay at Homo sapiens
3.6.1.29 6.8
-
 assay at Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.29 0.0021
-
 P1,P3-bis(5'-adenosyl) triphosphate pH 6.8, 30°C, in presence of Mg2+ Arabidopsis thaliana
3.6.1.29 0.0071
-
 adenosine 5'-phosphosulfate pH 6.8, 30°C Arabidopsis thaliana
3.6.1.29 0.0136
-
 adenosine 5'-phosphosulfate pH 6.8, 30°C Homo sapiens
3.6.1.29 0.018
-
 adenosine 5'-O-(gamma-fluoromonophosphate) pH 6.8, 30°C Arabidopsis thaliana
3.6.1.29 0.018
-
 P1,P3-bis(5'-adenosyl) triphosphate pH 6.8, 30°C, in presence of Mg2+ Homo sapiens
3.6.1.29 0.11
-
 AMP pH 6.8, 30°C Homo sapiens
3.6.1.29 0.113
-
 AMP pH 6.8, 30°C Arabidopsis thaliana
3.6.1.29 0.14
-
 ADP pH 6.8, 30°C Arabidopsis thaliana
3.6.1.29 0.145
-
 ATP pH 6.8, 30°C Arabidopsis thaliana
3.6.1.29 0.235
-
 adenosine 5'-O-(gamma-fluoromonophosphate) pH 6.8, 30°C, in presence of Mg2+ Arabidopsis thaliana