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Literature summary extracted from

  • Hwang, S.R.; Hook, V.
    Zinc regulation of aminopeptidase B involved in neuropeptide production (2008), FEBS Lett., 582, 2527-2531.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.6 Zn2+ low levels of Zn2+ at 0.00025-0.002 mM result in some activation of AP-B activity up to 25-40% above controls (without Zn2+) Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.6 expressed in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 Zn2+ Zn2+ inhibits AP-B reversibly at micromolar concentrations (0.005-0.05 mM), AP-B with 0.25 Zn2+ becomes susceptible to degradation by trypsin suggesting that Zn2+ alters enzyme conformation, complete inhibition occurs at 0.050-0.080 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.6 secretory vesicle
-
 Rattus norvegicus 99503
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.6 74000
-
 in the absence of ZnCl2, SDS-PAGE, after incubation with trypsin Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 Ni-NTA column chromatography Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 Arg-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 aminopeptidase B
-
 Rattus norvegicus
3.4.11.6 Ap-B
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.6 0.006
-
 Zn2+ in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, at 37°C Rattus norvegicus