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Literature summary extracted from

  • Lacombe-Harvey, M.E.; Fukamizo, T.; Gagnon, J.; Ghinet, M.G.; Dennhart, N.; Letzel, T.; Brzezinski, R.
    Accessory active site residues of Streptomyces sp. N174 chitosanase: variations on a common theme in the lysozyme superfamily (2009), FEBS J., 276, 857-869.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expression of CsnN174 in Escherichia coli JM109. T45S chitosanase mutant introduced into Streptomyces lividans Streptomyces sp. N174

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 D40E lower activity than mutant D40G Streptomyces sp. N174
3.2.1.132 D40G reaction time is 20 min. It is distinguished from wild-type by a lower activity, without changing the mechanism of hydrolysis or the mode of interaction with substrate Streptomyces sp. N174
3.2.1.132 D40G/T45D reaction time is 100 min. It has 0.1% of wild-type specific activity when tested on chitosan substrate Streptomyces sp. N174
3.2.1.132 D40G/T45E has 0.03% of wild-type specific activity when tested on chitosan substrate Streptomyces sp. N174
3.2.1.132 D40N lower activity than mutant D40G Streptomyces sp. N174
3.2.1.132 E36A reaction time is 10 min Streptomyces sp. N174
3.2.1.132 E36A/D40G reaction time is 100 min. Kcat is ca. 18times lower than that of the single mutant D40G. Rate of (GlcN)6 degradation is enhanced by sodium azide Streptomyces sp. N174
3.2.1.132 E36D reaction time is 10 min Streptomyces sp. N174
3.2.1.132 E36N reaction time is 10 min Streptomyces sp. N174
3.2.1.132 E36Q reaction time is 10 min Streptomyces sp. N174
3.2.1.132 E36Q/D40G reaction time is 100 min. Kcat is more than 5times lower than that of the single mutant D40G Streptomyces sp. N174
3.2.1.132 T45E has a very low residual activity. Activity of this mutant can not be enhanced by sodium azide Streptomyces sp. N174
3.2.1.132 T45H results in a complete loss of activity Streptomyces sp. N174
3.2.1.132 T45S reaction time is 20 min. It is quite active, keeps ca. 71% of the specific activity of the wild-type enzyme Streptomyces sp. N174
3.2.1.132 V148T has 10% of wild-type activity when tested on chitosan substrate Streptomyces sp. N174

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 additional information extent of growth inhibition is dependent on chitosan concentration, average molecular mass, the pH of the medium and salt composition. Growth inhibition can be suppressed by the expression of CsnN174 in Escherichia coli JM109 Streptomyces sp. N174

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Streptomyces sp. N174 P33665
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 0.01
-
 mutant T45H Streptomyces sp. N174
3.2.1.132 0.011
-
 mutant T45E Streptomyces sp. N174
3.2.1.132 0.02
-
 mutant D40G/T45E Streptomyces sp. N174
3.2.1.132 0.06
-
 mutant D40G/T45D Streptomyces sp. N174
3.2.1.132 0.07
-
 mutant E36A/D40G Streptomyces sp. N174
3.2.1.132 0.3
-
 mutant E36Q/D40G Streptomyces sp. N174
3.2.1.132 1.6
-
 mutant D40G Streptomyces sp. N174
3.2.1.132 21.9
-
 mutant E36N Streptomyces sp. N174
3.2.1.132 29.1
-
 mutant E36D Streptomyces sp. N174
3.2.1.132 29.2
-
 mutant E36A Streptomyces sp. N174
3.2.1.132 37.6
-
 mutant T45S Streptomyces sp. N174
3.2.1.132 44.7
-
 mutant E36Q Streptomyces sp. N174
3.2.1.132 52.9
-
 wild-type Streptomyces sp. N174

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O catalyzes the hydrolysis of beta-1,4-glycosidic links Streptomyces sp. N174 chitosan-oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 CsnN174
-
 Streptomyces sp. N174

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.132 0.0167
-
 chitosan mutant D40G/T45D, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 0.024
-
 chitosan mutant E36A/D40G, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 0.0368
-
 chitosan mutant T45E, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 0.0683
-
 chitosan mutant E36Q/D40G, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 0.44
-
 chitosan mutant D40G, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 6.827
-
 chitosan mutant E36N, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 8.34
-
 chitosan mutant E36D, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 10.48
-
 chitosan mutant T45S, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 11.47
-
 chitosan mutant E36A, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 11.66
-
 chitosan mutant E36Q, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174
3.2.1.132 12.4
-
 chitosan wild-type, assayed as the amount of D-glucosamine released from chitosan Streptomyces sp. N174