Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pollegioni, L.; Piubelli, L.; Molla, G.
    Cholesterol oxidase: biotechnological applications (2009), FEBS J., 276, 6857-6870.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Arthrobacter sp.
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Mycobacterium sp.
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Nocardia rhodochrous
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Nocardia erythropolis
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Rhodococcus equi
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Schizophyllum commune
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Pseudomonas spp.
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Streptomyces violascens
1.1.3.6 agriculture recombinant expression in plants gives insect resistance uncultured Gammaproteobacteria bacterium
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Streptoverticillum cholesterolicum
1.1.3.6 agriculture recombinant expression in plants gives insect resistance Brevibacterium sterolicum
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Arthrobacter sp.
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Mycobacterium sp.
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Nocardia rhodochrous
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Nocardia erythropolis
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Rhodococcus equi
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Schizophyllum commune
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Pseudomonas spp.
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Streptomyces violascens
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool uncultured Gammaproteobacteria bacterium
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Streptoverticillum cholesterolicum
1.1.3.6 biotechnology biocatalysis, industrial steroid drug production, steroid production as diagnostic tool Brevibacterium sterolicum
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Arthrobacter sp.
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Mycobacterium sp.
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Nocardia rhodochrous
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Nocardia erythropolis
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Rhodococcus equi
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Schizophyllum commune
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Pseudomonas spp.
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Streptomyces violascens
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples uncultured Gammaproteobacteria bacterium
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Streptoverticillum cholesterolicum
1.1.3.6 diagnostics analytic tool for determining cholesterol in various samples Brevibacterium sterolicum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.6 additional information engineered ChOx to improve activity and alter specificity Rhodococcus equi
1.1.3.6 additional information engineered ChOx to improve activity and alter specificity Streptomyces violascens
1.1.3.6 additional information engineered ChOx to improve activity and alter specificity Streptoverticillum cholesterolicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.6 extracellular
-
 Schizophyllum commune
-
-
1.1.3.6 extracellular
-
 Pseudomonas spp.
-
-
1.1.3.6 extracellular
-
 Streptomyces violascens
-
-
1.1.3.6 extracellular
-
 Streptoverticillum cholesterolicum
-
-
1.1.3.6 extracellular
-
 Brevibacterium sterolicum
-
-
1.1.3.6 intracellular
-
 Arthrobacter sp. 5622
-
1.1.3.6 intracellular
-
 Mycobacterium sp. 5622
-
1.1.3.6 intracellular
-
 Nocardia rhodochrous 5622
-
1.1.3.6 intracellular
-
 Nocardia erythropolis 5622
-
1.1.3.6 intracellular
-
 Rhodococcus equi 5622
-
1.1.3.6 membrane
-
 Arthrobacter sp. 16020
-
1.1.3.6 membrane
-
 Mycobacterium sp. 16020
-
1.1.3.6 membrane
-
 Nocardia rhodochrous 16020
-
1.1.3.6 membrane
-
 Nocardia erythropolis 16020
-
1.1.3.6 membrane
-
 Rhodococcus equi 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Arthrobacter sp.
-
-
-
1.1.3.6 Brevibacterium sterolicum P22637
-
-
1.1.3.6 Mycobacterium sp.
-
-
-
1.1.3.6 Nocardia erythropolis
-
-
-
1.1.3.6 Nocardia rhodochrous
-
-
-
1.1.3.6 Pseudomonas spp.
-
-
-
1.1.3.6 Rhodococcus equi
-
 high levels of ChOx activity, significant similarities to putative ChOxs encoded by Mycobacterium tuberculosis and Mycobacterium leprae
-
1.1.3.6 Schizophyllum commune
-
-
-
1.1.3.6 Streptomyces violascens
-
-
-
1.1.3.6 Streptoverticillum cholesterolicum
-
-
-
1.1.3.6 uncultured Gammaproteobacteria bacterium
-
 Y-134
-
1.1.3.6 uncultured Gammaproteobacteria bacterium Y-134
-
 Y-134
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 additional information
-
 comparison of relative activity of ChOx from different sources Rhodococcus equi
1.1.3.6 additional information
-
 comparison of relative activity of ChOx from different sources uncultured Gammaproteobacteria bacterium
1.1.3.6 additional information
-
 comparison of relative activity of ChOx from different sources Brevibacterium sterolicum
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Arthrobacter sp.
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Mycobacterium sp.
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Nocardia rhodochrous
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Nocardia erythropolis
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Rhodococcus equi
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Schizophyllum commune
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Pseudomonas spp.
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Streptomyces violascens
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) uncultured Gammaproteobacteria bacterium
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Streptoverticillum cholesterolicum
1.1.3.6 additional information
-
 comparison of the effect of detergents and solvents on ChOx activity and stability (from different sources) Brevibacterium sterolicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 7-keto-cholesterol + O2
-
 Rhodococcus equi ?
-
 ?
1.1.3.6 7-keto-cholesterol + O2
-
 uncultured Gammaproteobacteria bacterium ?
-
 ?
1.1.3.6 7-keto-cholesterol + O2
-
 Brevibacterium sterolicum ?
-
 ?
1.1.3.6 7-keto-cholesterol + O2
-
 uncultured Gammaproteobacteria bacterium Y-134 ?
-
 ?
1.1.3.6 7alpha-hydroxy-cholesterol + O2
-
 Rhodococcus equi 7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 7alpha-hydroxy-cholesterol + O2
-
 uncultured Gammaproteobacteria bacterium 7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 7alpha-hydroxy-cholesterol + O2
-
 Brevibacterium sterolicum 7alpha-hydroxy-cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 7beta-hydroxy-cholesterol + O2
-
 Rhodococcus equi 7beta-hydroxy-cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 7beta-hydroxy-cholesterol + O2
-
 uncultured Gammaproteobacteria bacterium 7beta-hydroxy-cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 7beta-hydroxy-cholesterol + O2
-
 Brevibacterium sterolicum 7beta-hydroxy-cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2
-
 Rhodococcus equi ?
-
 ?
1.1.3.6 beta-sitosterol + O2
-
 uncultured Gammaproteobacteria bacterium ?
-
 ?
1.1.3.6 beta-sitosterol + O2
-
 Brevibacterium sterolicum ?
-
 ?
1.1.3.6 beta-sitosterol + O2
-
 uncultured Gammaproteobacteria bacterium Y-134 ?
-
 ?
1.1.3.6 cholestanol + O2
-
 Rhodococcus equi ?
-
 ?
1.1.3.6 cholestanol + O2
-
 uncultured Gammaproteobacteria bacterium ?
-
 ?
1.1.3.6 cholestanol + O2
-
 Brevibacterium sterolicum ?
-
 ?
1.1.3.6 cholesterol + O2
-
 Arthrobacter sp. cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Mycobacterium sp. cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Nocardia rhodochrous cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Nocardia erythropolis cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Rhodococcus equi cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Schizophyllum commune cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Pseudomonas spp. cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Streptomyces violascens cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 uncultured Gammaproteobacteria bacterium cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Streptoverticillum cholesterolicum cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 uncultured Gammaproteobacteria bacterium Y-134 cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 ergosterol + O2
-
 Rhodococcus equi ?
-
 ?
1.1.3.6 ergosterol + O2
-
 uncultured Gammaproteobacteria bacterium ?
-
 ?
1.1.3.6 ergosterol + O2
-
 Brevibacterium sterolicum ?
-
 ?
1.1.3.6 ergosterol + O2
-
 uncultured Gammaproteobacteria bacterium Y-134 ?
-
 ?
1.1.3.6 pregnenolone + O2
-
 Rhodococcus equi ?
-
 ?
1.1.3.6 pregnenolone + O2
-
 uncultured Gammaproteobacteria bacterium ?
-
 ?
1.1.3.6 pregnenolone + O2
-
 Brevibacterium sterolicum ?
-
 ?
1.1.3.6 pregnenolone + O2
-
 uncultured Gammaproteobacteria bacterium Y-134 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.6 3beta-hydroxysterol oxidase
-
 Arthrobacter sp.
1.1.3.6 3beta-hydroxysterol oxidase
-
 Mycobacterium sp.
1.1.3.6 3beta-hydroxysterol oxidase
-
 Nocardia rhodochrous
1.1.3.6 3beta-hydroxysterol oxidase
-
 Nocardia erythropolis
1.1.3.6 3beta-hydroxysterol oxidase
-
 Rhodococcus equi
1.1.3.6 3beta-hydroxysterol oxidase
-
 Schizophyllum commune
1.1.3.6 3beta-hydroxysterol oxidase
-
 Pseudomonas spp.
1.1.3.6 3beta-hydroxysterol oxidase
-
 Streptomyces violascens
1.1.3.6 3beta-hydroxysterol oxidase
-
 uncultured Gammaproteobacteria bacterium
1.1.3.6 3beta-hydroxysterol oxidase
-
 Streptoverticillum cholesterolicum
1.1.3.6 3beta-hydroxysterol oxidase
-
 Brevibacterium sterolicum
1.1.3.6 cholesterol oxidase
-
 Arthrobacter sp.
1.1.3.6 cholesterol oxidase
-
 Mycobacterium sp.
1.1.3.6 cholesterol oxidase
-
 Nocardia rhodochrous
1.1.3.6 cholesterol oxidase
-
 Nocardia erythropolis
1.1.3.6 cholesterol oxidase
-
 Rhodococcus equi
1.1.3.6 cholesterol oxidase
-
 Schizophyllum commune
1.1.3.6 cholesterol oxidase
-
 Pseudomonas spp.
1.1.3.6 cholesterol oxidase
-
 Streptomyces violascens
1.1.3.6 cholesterol oxidase
-
 uncultured Gammaproteobacteria bacterium
1.1.3.6 cholesterol oxidase
-
 Streptoverticillum cholesterolicum
1.1.3.6 cholesterol oxidase
-
 Brevibacterium sterolicum
1.1.3.6 ChOx
-
 Arthrobacter sp.
1.1.3.6 ChOx
-
 Mycobacterium sp.
1.1.3.6 ChOx
-
 Nocardia rhodochrous
1.1.3.6 ChOx
-
 Nocardia erythropolis
1.1.3.6 ChOx
-
 Rhodococcus equi
1.1.3.6 ChOx
-
 Schizophyllum commune
1.1.3.6 ChOx
-
 Pseudomonas spp.
1.1.3.6 ChOx
-
 Streptomyces violascens
1.1.3.6 ChOx
-
 uncultured Gammaproteobacteria bacterium
1.1.3.6 ChOx
-
 Streptoverticillum cholesterolicum
1.1.3.6 ChOx
-
 Brevibacterium sterolicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Arthrobacter sp.
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Mycobacterium sp.
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Nocardia rhodochrous
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Nocardia erythropolis
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Rhodococcus equi
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Schizophyllum commune
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Pseudomonas spp.
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Streptomyces violascens
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Streptoverticillum cholesterolicum
1.1.3.6 FAD both as a covalently or noncovalently bound cofactor Brevibacterium sterolicum
1.1.3.6 FMN covalently bound uncultured Gammaproteobacteria bacterium